Study on chemical structure and mechanism of soil-phase electron mediator enhancing dechlorination activity of anaerobic microorganisms

土相电子介体增强厌氧微生物脱氯活性的化学结构及机理研究

• 批准号: 23310055
• 负责人: KATAYAMA arata
• 金额: $ 12.65万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23310055/
• 关键词: 固体電子メディエータ; 脱ハロゲン反応; 芳香族ハロゲン化合物; 酸化還元中心; 細胞外電子伝達系; ヒューミン; ペンタクロロフェノール; 四臭化ビスフェノールA; 嫌気脱ハロゲン反応; 嫌気的脱塩素反応; キノン構造; 化学的安定性; 微生物付着; 腐植物質; 電子メディエータ; 還元的脱ハロゲン反応; 13C-NMR; サイクリックボルタンメトリ; ESR; FT-IR

Solid-phase humin, a fraction of humic substances in soil and sediment which is not soluble in any pH condition, was found to function as external electron mediator for the anaerobic consortium reductively-dechlorinating pentachlorophenol. The humic acids extracted from the same source did not support the pentachlorophenol dechlorination. The redox-reacting centers of humin were suggested to be quinone structure or iron-organic acid chelating complex, based on the analyses using FT-IR, NMR, ESR, cyclic voltammetry. It was suggested that a novel Dehalobacter sp., requiring reduced-form humin as electron donor and pentachlorophenol as electron acceptor for the organohalide respiration, was present in the enriched consortium. It was considered that the electrons transfer from humin to microbial cells directly.

固相humin是土壤和沉积物中腐殖质的一部分，在任何pH条件下都不可溶，可作为厌氧财团还原性五氯苯酚的外部电子介体。从同一来源提取的腐殖酸不支持戊二醇脱氯。基于使用FT-IR，NMR，ESR，环状伏安法的分析，建议humin的氧化还原反应中心是喹酮结构或铁酸螯合复合物。有人提出，在富集的联盟中，存在一种新型的脱脂杆菌，需要减少形式的humin作为电子供体和五氯苯酚作为有机甲基呼吸的电子受体。被认为电子从humin转移到微生物细胞。

项目成果

Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions

铁还原条件下富集培养物中的厌氧 4-氯酚矿化

• DOI: 10.1016/j.jbiosc.2014.04.007
• 发表时间: 2014
• 期刊: Journal of Bioscience and Bioengineering
• 影响因子: 2.8
• 作者: Zhiling Li;Daisuke Suzuki;Chunfang Zhang;Suyin Yang;Jun Nan;Naoko Yoshida;Aijie Wang;Arata Katayama
• 通讯作者: Arata Katayama

Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity

• DOI: 10.1007/s11274-013-1310-8
• 发表时间: 2013-03
• 期刊: World Journal of Microbiology and Biotechnology
• 影响因子: 4.1
• 作者: D. Suzuki;D. Baba;V. Satheeja Santhi;R. D. Jebakumar Solomon;A. Katayama

A novel Dehalobacter sp. is involved in anaerobic dechlorination of Trichlorophenol (TCP)

一种新型脱盐杆菌 sp。

• 发表时间: 2012
• 作者: 杉山千尋;古武弥一郎;津山由美;奥田勝博;太田 茂;Zhiling Li
• 通讯作者: Zhiling Li

The microbial spatial distribution in a long-term maintained biobar rier for pentachlorophenol (PCP) anaerobic mineralization

五氯苯酚（PCP）厌氧矿化长期维持的生物屏障中微生物的空间分布

• 发表时间: 2011
• 作者: Zhiling LI;Yasushi INOUE;Daisuke SUZUKI;Arata KATAYAMA
• 通讯作者: Arata KATAYAMA

Polyphasic characterization of two anaerobic microbial consortia dechlorinating wide spectra of phenols with lower levels of chlorination

两种厌氧微生物群落以较低氯化水平对广谱酚进行脱氯的多相表征

• 发表时间: 2012
• 作者: Chunfang ZHANG ;Daisuke SUZUKI;Zhiling LI;Lizhen YE;Arata KATAYAMA
• 通讯作者: Arata KATAYAMA