Development of education movies demonstrating real-time biophysical phenomena by two photon laser microscopy

开发教育电影，通过双光子激光显微镜展示实时生物物理现象

• 批准号: 23300284
• 负责人: MIZOGUCHI Akira
• 金额: $ 12.15万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300284/
• 关键词: 2光子レーザー顕微鏡; 生体内細胞観察; GFPマウス; iPS細胞; 病気の可視化; 治療効果の可視化; 生命科学実感教材の開発; 超早期癌診断・治療; 二光子レーザー顕微鏡; 命科学実感教材の開発; 癌の診断; 癌の治療; 神経疾患の診断

I have engaged in development of education movies demonstrating real-time biophysical phenomena in vivo and in vitro by two photon laser microscopy. Two photon laser microscopy is a new method based on two photon excitation which generates new photons inside the animal body for visualizing real-time images of cells. These real-time movies demonstrating dynamic phenomena occurring inside our body probably enhance the understanding of students studying biological science. In this study I have obtained the real-time images of normal cells in gastrointestinal tracts, liver, pancreas, kidney, brain, and vessels. I have also observed the real-time images of cancer cells in intestines, which gives me an opportunity to develop a novel method to identify very small cancers at the ultra-early stages. The new method has been submitted as an international patent under the financial support from JST. The education movies obtained in this study will be opened in a free internet service.

我从事教育电影的发展，展示了体内实时生物物理现象，并通过两个光子激光显微镜在体外进行了体外。两个光子激光显微镜是一种基于两个光子激发的新方法，该方法在动物体内产生新的光子，以可视化细胞的实时图像。这些展示动态现象发生的实时电影可能会增强对学习生物科学的学生的理解。在这项研究中，我获得了胃肠道，肝脏，胰腺，肾脏，大脑和血管中正常细胞的实时图像。我还观察到肠道中癌细胞的实时图像，这使我有机会开发一种新型方法，可以在超前的阶段识别非常小的癌症。在JST的财政支持下，新方法已作为国际专利提交。在这项研究中获得的教育电影将在免费的互联网服务中开业。

项目成果

In vivo real-time two-photon microscopic imaging of platelet aggregation induced by selective laser irradiation to the endothelium created in the beta-actin-green fluorescent protein transgenic mice

• DOI: 10.1007/s11239-011-0600-y
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF THROMBOSIS AND THROMBOLYSIS
• 影响因子: 4
• 作者: Koike, Yuhki;Tanaka, Koji;Kusunoki, Masato
• 通讯作者: Kusunoki, Masato

Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse

• DOI: 10.1093/hmg/ddr400
• 发表时间: 2011-12-01
• 期刊: HUMAN MOLECULAR GENETICS
• 影响因子: 3.5
• 作者: Kuroda, Keisuke;Yamada, Shinnosuke;Kaibuchi, Kozo
• 通讯作者: Kaibuchi, Kozo

Intravital Three-Dimensional Dynamic Pathology of Experimental Colitis in Living Mice Using Two-Photon Laser Scanning Microscopy

• DOI: 10.1007/s11605-011-1632-5
• 发表时间: 2011-07
• 期刊: Journal of Gastrointestinal Surgery
• 影响因子: 3.2
• 作者: Y. Morimoto;Koji Tanaka;Y. Toiyama;Y. Inoue;T. Araki;K. Uchida;K. Kimura;A. Mizoguchi;M. Kusunoki

In vivo optical pathology of paclitaxel efficacy on the peritoneal metastatic xenograft model of gastric cancer using two-photon laser scanning microscopy

使用双光子激光扫描显微镜观察紫杉醇对胃癌腹膜转移异种移植模型的体内光学病理学疗效

• DOI: 10.1007/s10120-013-0334-y
• 发表时间: 2014
• 期刊: Gastric Cancer
• 影响因子: 7.4
• 作者: Shimura T;Tanaka K;Toiyama Y;Okigami M;Ide S;Kitajima T;Kondo S;Saigusa S;Ohi M;Araki T;Inoue Y;Uchida K;Mohri Y;Mizoguchi A;Kusunoki M
• 通讯作者: Kusunoki M

多光子レーザ診断治療装置

多光子激光诊断治疗仪

• 发表时间: 2013