Study on the physiological function of bombyxin

家蚕素的生理功能研究

• 批准号: 04640655
• 负责人: MIZOGUCHI Akira
• 金额: $ 1.34万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640655/
• 关键词: Bombyx mori; Insulin-like peptide; Bombyxin; Fluoroimmunoassay; Metamorphosis; Metabolism; Trehalase; glycogen; 分泌動態; 時間分解蛍光免疫測定法; 糖代謝; 脂質代謝; 時間分解螢光免疫測定法; 血糖低下作用; トレハロース; 微量定量法; モノクローナル抗体

1. We produced many kinds of mouse monoclonal antibodies against synthetic bombyxin-II and developed a time-resolved fluoroimmunoassay (TR-FIA) for bombyxin. This assay method is superior to the conventional radioimmunoassay in sensitivity, simplicity, safety and time-saving.2. Using TR-FIA,we examined the changes in bombyxin titer in hemolymph during larva-pupa-adult development of the silkmoth, Bombyx mori. The titer was low in the larval stage, while high in the early and middle pupal stages. A steep rise in the titer was observed immediately after eclosion only in males. Hemolymph lipid also increased after eclosion only in males, suggesting a possibility that bombyxin is involved in the regulation of lipid metabolism.3. We investigated the physiological function of bombyxin in Bombyx larvae and obtained the following results.1) Bombyxin has a hypotrehalosemic activity.2) Bombyxin increases the trehalase activity in the midgut and integument (including muscles).3) Bombyxin has no effect on trehalose synthase activity in the fat body.4) Bombyxin decreases glycogen content in the fat body.These results suggested that bombyxin regulates sugar and lipid metabolism and that bombyxin plays an important role (s) in the process of metamorphosis.

1。我们对合成Bombyxin-II产生了多种小鼠单克隆抗体，并为Bombyxin开发了一种时间分辨的氟免疫测定法（TR-FIA）。在灵敏度，简单性，安全性和节省时间里，这种测定方法优于常规的放射免疫测定法。2。使用TR-FIA，我们检查了丝绸史密斯（Bombyx Mori）的幼虫 - 帕帕（Bombyx Mori）幼虫形成期间血淋巴中Bombyxin滴度的变化。在幼虫阶段的滴度很低，而在早期和中型阶段高。仅在雄性中，直接观察到滴度的急剧上升。仅在男性中进行ECLOSIN后，血淋巴脂质也会增加，这表明Bombyxin可能参与调节脂质代谢的调节3。我们研究了Bombyx幼虫中Bombyxin的生理功能，并获得了以下结果。1）Bombyxin具有低透明度的活性。2）Bombyxin在中肠和外线中增加了Trehalase的活性（包括肌肉）。在脂肪体中，这些结果表明，Bombyxin调节糖和脂质代谢，而Bombyxin在变态过程中起着重要作用。

项目成果

三枝 弘尚: "Changes in the titer of bombyxin-immunoreactive material in hemolymph during the postembryonic development of the silkmoth Bombyx mori." Development Growth and Differentiation. 34. 595-605 (1992)

Hironaka Saegusa：“家蚕胚胎后发育过程中血淋巴中蚕素免疫反应物质滴度的变化。” 34. 595-605 (1992)。

J.-d.Dai, et al.: "Immunoreactivity of neurosecretorygranules in the brain-retrocerebral complex of Manduca sexta to heterologous antibodies against Bombyx prothoracicotropic hormone and bombyxin." Invertebrate Reproduction and Development. 26. 187-196 (1

J.-d.Dai 等人：“天蛾脑-脑后复合体中的神经分泌颗粒对针对家蚕促胸腺激素和家蚕素的异源抗体的免疫反应性。”

溝口 明(分担): "Perspectives in Comparative Endocrinology(K.G.Davey他編)" Research Journals Division of the National Research council of Canada.(印刷中), (1994)

Akira Mizoguchi（撰稿人）：“比较内分泌学的观点（由 K.G. Davey 等人编辑）”加拿大国家研究委员会研究期刊部（正在出版），（1994 年）。

三枝 弘尚: "Change in the titer of bombyxin-immunoreactive material in hemolymph during the postembryonic development of the silkmoth Bombyx mori." Development Growth and Differentiation. 34. 595-605 (1992)

Hironaka Saegusa：“家蚕胚胎后发育过程中血淋巴中家蚕素免疫反应物质滴度的变化。” 34. 595-605 (1992)。

溝口 明 (分担): "Perspectives in Comparative Endocrinology(K.G.Davey 他編)" National Research Council of Canada, 654,(5) (1994)

Akira Mizoguchi（撰稿人）：“比较内分泌学的观点（K.G.Davey 等编辑）”加拿大国家研究委员会，654,(5) (1994)