Periodontal regeneration by BMP application to root surface and scaffold of periodontal ligament

BMP应用于牙根表面和牙周膜支架的牙周再生

• 批准号: 16390610
• 负责人: SUGAYA Tsutomu
• 金额: $ 6.29万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390610/
• 关键词: Periodontal regeneration; BMP-2; Collagen hydrogel; Root surface conditioning; Scaffold; Hard tissue formation; Root resorption; Coupling; コラーゲン; 1壁性骨欠損

The aim of the first experiment was to evaluate the effect of different dentin conditionings followed rhBMP-2 application on dentin surface. Dentin blocks were demineralized with EDTA, tetracycline-HCI or citric acid solutions for 3 minutes, and were soaked with rhBMP-2 solution. Periodontal ligament cells were seeded on each dentin blocks, and ALP activity of attachment cells were assessed. The results showed that the groups of EDTA demineralization had higher ALP activity. In the second experiment, each dentin blocks were transplanted into palatal connective tissue of rats. The histological examinations showed that the groups of EDTA demineralization and rhBMP-2 application had significantly more hard tissue formation than any other groups. In the third experiment, dentin blocks were demineralized with EDTA and rhBMP-2 applied, and then implanted into palatal connective tissue of rats. Bisphosponate was administered to rats for osteoclast suppression. The results showed that hard tis … More sue formation and dentin resorption were decreased. It suggested that coupling system between osteoblast and osteoclast may play an important role in hard tissue formation on dentin surface. The aim of fourth study was to evaluate periodontal regeneration in dehiscence model after EDTA demineralization, application of BMP-2 solution and collagen hydrogel to root dentin surfaces. New bone formation was significantly greater than any other group. Then, periodontal regeneration in 1-wall infrabony defects was evaluated. New bone and new cementum formation was significantly greater than in the control group. New cementum isolated from original cementum was frequently observed in the coronal area, and it might be formed by gingival connective tissue. These results suggest that new aspects of the regenerative process are introduced.It was concluded that the application of BMP-2 solution and collagen hydrogel to root dentin surface was conducive to periodontal tissue regeneration. This procedure may be effective regenerative therapy for large periodontal defects. Less

第一个实验的目的是评估rhBMP-2应用后不同牙本质处理对牙本质表面的影响，牙本质块用EDTA、四环素-HCl或柠檬酸溶液脱矿3分钟，并用rhBMP-2溶液浸泡。将牙周膜细胞接种到每个牙本质块上，并评估附着细胞的ALP活性，结果显示EDTA组有脱矿。在第二个实验中，将每个牙本质块移植到大鼠的腭结缔组织中，组织学检查显示，EDTA脱矿和rhBMP-2应用组的硬组织形成明显多于其他组。实验中，用EDTA和rhBMP-2对牙本质块进行脱矿，然后植入大鼠腭结缔组织中，给予大鼠破骨细胞。结果表明，硬组织形成和牙本质吸收减少，这表明成骨细胞和破骨细胞之间的耦合系统可能在牙本质表面硬组织形成中发挥重要作用。在 EDTA 脱矿后的开裂模型中，将 BMP-2 溶液和胶原水凝胶应用于牙根牙本质表面，新骨形成显着高于任何其他组。评估了 1 壁骨下缺损，新骨和新牙骨质的形成明显多于对照组，在冠状区域经常观察到与原始牙骨质分离的新牙骨质，并且它可能是由牙龈结缔组织形成的。结论是，将BMP-2溶液和胶原水凝胶应用于牙根牙本质表面有利于牙周组织再生，该过程可能是大面积牙周组织的有效再生治疗。牙周缺陷较少。

项目成果

BMP-2とコラーゲンハイドロゲルの歯根象牙質表面への塗布による歯周組織再生

将 BMP-2 和胶原水凝胶应用于牙根牙本质表面进行牙周组织再生

• 发表时间: 2006
• 作者: 石塚良介; 宮治裕史; 菅谷 勉; 川浪雅光
• 通讯作者: 川浪雅光

Bisphosphonate injection suppresses cementun-like tissue formation and dentin resorption induced by BMP-2 application

双膦酸盐注射抑制 BMP-2 应用诱导的牙骨质样组织形成和牙本质吸收

• 发表时间: 2006
• 作者: Hongo T; Miyaji H; Kawamura N; Tenkumo T; Ohtani K; Tokunga K; Sugaya T; Kawanami M
• 通讯作者: Kawanami M

Periodontal tissue reaction on BMP application to root dentin surface

BMP应用于根牙本质表面的牙周组织反应

• 发表时间: 2006
• 作者: Miyaji H; Sugaya T; Ibe K; Ishizuka R; Tokunaga K; Kawanami M.
• 通讯作者: Kawanami M.

Periodontal regeneration by BMP-2 solution and collagen hydrogel application to root dentin surface.

• DOI: 10.2329/perio.48.255
• 发表时间: 2024-09-13
• 期刊: Nihon Shishubyo Gakkai Kaishi (journal of The Japanese Society of Periodontology)
• 作者: Ryousuke Ishizuka;H. Miyaji;T. Sugaya;M. Kawanami
• 通讯作者: M. Kawanami

Effect of BMP-2 application to root dentin surface on periodontal regeneration in 1-wall ifrabony defects

BMP-2 应用于根牙本质表面对 1 壁 ifrabony 缺损牙周再生的影响

• 发表时间: 2007
• 作者: Kato A; Miyaji H; Sugaya T; Kawanmi M
• 通讯作者: Kawanmi M