Flower form alteration in monocot ornamental plants by genetic transformation

通过遗传转化改变单子叶观赏植物的花形

基本信息

批准号：
16380024
负责人：
NAKANO Masaru
金额：
$ 10.05万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

In the present study, the following experiments were carried out in order to alter flower form in monocot ornamentals by genetic transformation.1. Establishment of genetic transformation systemsAn efficient and reproducible system for Agrobacterium-mediated transformation was established in Tricyrtis hirta. In addition, long-term and stable expression of a foreign gene was confirmed in transgenic plants of Agapanthus praecox ssp. orientalis and T. hirta.2. Isolation and characterization of MADS-box genes involved in the ABC modelVarious MADS-box genes, mainly the class B genes, were isolated from A. praecox ssp. orientalis, Muscari armeniacum, Trillium camtschatcense, Tricyrtis affinis and Alstroemeria ligtu. Expression of the class B genes was observed in both inner and outer tepals, which supports the modified ABC model.3. Isolation and characterization of cycloidea (CFC)-like genesCYC-like genes were isolated from two Alstroemeria species. Expression analyses of these genes indicate that the floral zygomorphy in monocot plants is controlled in a similar manner to dicot plants.4. Genetic transformation with MADS-box genes involved in the ABC modelThe class B genes isolated from A. praecox ssp. orientalis were introduced to each of T.hirta, Nicotiana tabacum and Lactuca sativa by Agrobacterium-mediated transformation. For all the three species, some transgenic plants produced flowers with apparent morphological alterations. These results indicate a possibility of molecular breeding for flower form alteration by genetic transformation with the class B MADS-box gene(s) of heterologous plant species.

在本研究中，为了通过遗传转化改变单子叶观赏植物的花形，进行了以下实验： 1．遗传转化系统的建立在Tricyrtis hirta 中建立了高效且可重复的农杆菌介导转化系统。此外，在百子莲转基因植物中证实了外源基因的长期稳定表达。侧柏和T. hirta.2。 ABC 模型中涉及的 MADS-box 基因的分离和表征从 A. praecox ssp 中分离出各种 MADS-box 基因，主要是 B 类基因。侧柏、Muscari ameniacum、延龄草、Tricyrtis affinis 和 Alstroemeria ligtu。 B类基因在内、外花被片中均有表达，支持了改进的ABC模型。 3． CYC 样基因的分离和表征从两个六出花属物种中分离出CYC 样基因。这些基因的表达分析表明，单子叶植物花对称性的控制方式与双子叶植物相似。4． ABC 模型中涉及的 MADS-box 基因的遗传转化从 A. praecox ssp. 中分离出 B 类基因。通过农杆菌介导的转化将侧柏引入T.hirta、烟草和莴苣中的每一种中。对于所有这三个物种，一些转基因植物产生的花朵具有明显的形态变化。这些结果表明通过使用异源植物物种的B类MADS-box基因进行遗传转化来改变花形的分子育种的可能性。