Development of the affinity matrix for comprehensive analysis of ligand- binding proteins

开发用于综合分析配体结合蛋白的亲和矩阵

• 批准号: 22710219
• 负责人: KURAMOCHI Kouji
• 金额: $ 2.08万
• 依托单位: Kyoto Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22710219/
• 关键词: ケミカルバイオロジー; アフィニティー精製; ファージディスプレイ; 固相合成用樹脂; マンノース; コンカナバリンA; 生体分子; 生理活性; 蛋白質; 有機化学

Bioactive compounds such as drugs and toxins generally show their activities by interacting with their specific receptors. In this study, we explored the binding proteins of perfluorooctanesulfonic acid, which is a man-made fluorosurfactant and global pollutant, and apigenin, which is a natural product belonging to the flavone. We also developed a novel affinity matrix for purifying the binding proteins of bioactive compounds.

诸如药物和毒素之类的生物活性化合物通常通过与特定受体相互作用来显示其活性。在这项研究中，我们探索了一种人为的荧光表层活性剂和全球污染物和阿哌替宁，它是属于黄酮的天然产物。我们还开发了一种新型的亲和力基质，用于纯化生物活性化合物的结合蛋白。

项目成果

Synthetic and Structure-Activity Relationship Studies on Bioactive Natural Products

• DOI: 10.1271/bbb.120884
• 发表时间: 2013-03-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Kuramochi, Kouji

光固定化とファージディスプレイ法を融合した薬剤結合タンパク質の探索

结合光固定化和噬菌体展示方法寻找药物结合蛋白

• 发表时间: 2011
• 作者: Takano N;Yamamoto T;Adachi T;and Suematsu M;倉持 幸司
• 通讯作者: 倉持 幸司

Innovative approach to identify cellular targets of the anti-inflammatory flavonoid apigenin

识别抗炎类黄酮芹菜素细胞靶标的创新方法

• 发表时间: 2011
• 作者: Kajimura M;Fukuda R;Bateman RM;Yamamoto T;and Suematsu M;Daniel Arango
• 通讯作者: Daniel Arango

Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets

• DOI: 10.1073/pnas.1303726110
• 发表时间: 2013-06-11
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Arango, Daniel;Morohashi, Kengo;Doseff, Andrea I.
• 通讯作者: Doseff, Andrea I.

Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen

通过 T7 噬菌体显示屏探索全氟辛烷磺酸的结合蛋白

• DOI: 10.1016/j.bmc.2012.05.016
• 发表时间: 2012
• 期刊: Bioorg. Med. Chem.
• 作者: Miyano Y;Tsukuda S;Sakimoto I;Takeuchi R;Shimura S;Takahashi N;Kusayanagi T;Takakusagi Y;Okado M;Matsumoto Y;Takakusagi K;Takeuchi T;Kamisuki S;Nakazaki A;Ohta K;Miura M;Kuramochi K;Mizushina Y;Kobayashi S;Sugawara F;Sakaguchi K.
• 通讯作者: Sakaguchi K.