Thermotolerance through isoprene emission and the novel mechanism of decrease in leaf surface temperature in plants

植物异戊二烯释放的耐热性和降低叶面温度的新机制

• 批准号: 22657014
• 负责人: YAZAKI Kazufumi
• 金额: $ 1.84万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657014/
• 关键词: 環境応答; イソプレン; 高温耐性; プラスチド; MEP経路; 揮発性有機化合物; 植物由来揮発性有機化合物; 葉面温度低下; 非メバロン酸経路

Mechanism of thermotolerance by expressing isoprene synthase(IspS) in Arabidopsis thaliana has been studied, in which transgenic plants were treated at 60℃for 2. 5 hr. We compared various endogenous plant hormone contents between wild type and IspS-expressing Arabidopsis systematically, but could find significant difference. During the analysis we found that the tolerance to drastic and short time heat shock seems to be different to recovery rate of treated plants on cultivation at 23℃for a week. Considering with a recent report on the tolerance of IspS-expressing tobacco plant to ozone stress, it is suggested that the thermotolerance we observed may be due to the depletion of reactive oxygen species raised after heat shock treatment in plastids by isoprene, which actually functions as OH radical scavenger in atomosphere.

研究了拟南芥表达异戊二烯合酶(IspS)的耐热性机制，其中转基因植物在60℃下处理2. 5小时，我们系统地比较了野生型和表达IspS的拟南芥之间的各种内源植物激素含量。在分析过程中，我们发现处理后的植物对剧烈和短时间热激的耐受性似乎与在23℃下培养的恢复率不同。考虑到最近关于表达 IspS 的烟草植物对臭氧胁迫的耐受性的报告，表明我们观察到的耐热性可能是由于异戊二烯热激处理后质体中产生的活性氧的消耗所致。实际上在大气中起着 OH 自由基清除剂的作用。

项目成果

植物のイソプレノイド単位DMAPPを巡る分泌型低分子の代謝生理

植物类异戊二烯单元 DMAPP 周围分泌小分子的代谢生理学

• 发表时间: 2011
• 作者: Ichinomiya M;Yoshikawa S;Takaichi S;Kamiya M;Ohki K;Kuwata A;矢崎一史
• 通讯作者: 矢崎一史

A Tolerance Gene for Prenylated Flavonoid Encodes a 26S Proteasome Regulatory Subunit in Sophora flavescens

苦参中异戊二烯化类黄酮耐受基因编码 26S 蛋白酶体调节亚基

• DOI: 10.1271/bbb.100665
• 发表时间: 2011
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Shitan;N;et al
• 通讯作者: et al

Production of prenylated flavonoids in tomato fruits expressing a prenyltransferase gene from Streptomyces coelicolor A3(2)

• DOI: 10.1111/j.1438-8677.2010.00409.x
• 发表时间: 2011-03-01
• 期刊: PLANT BIOLOGY
• 影响因子: 3.9
• 作者: Koeduka, T.;Shitan, N.;Yazaki, K.
• 通讯作者: Yazaki, K.

イソプレン放出による高温耐性機構の解析

异戊二烯释放的耐高温机理分析

• 发表时间: 2010
• 作者: Ichinomiya M;Yoshikawa S;Takaichi S;Kamiya M;Ohki K;Kuwata A;矢崎一史;桑田晃;馬場奈央登
• 通讯作者: 馬場奈央登