Detection and observation of oxidative damage for the improvement of high-LET radiation therapy

氧化损伤的检测和观察以改善高LET放射治疗

• 批准号: 22611015
• 负责人: ITO Atsushi
• 金额: $ 2.83万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22611015/
• 关键词: 高LET放射線; 間接作用; ラジカル; 重粒子; DNA酸化損傷; 免疫染色法; 8-OHdG; 酸素効果; トラック構造; DNA薄膜; クラスター損傷; 蛍光抗体法

In order to visualize the distribution of a penumbra, a low LET region around ion tracks, we have developed the methods to image the distribution of 8-OHdG, a radiation product resulting from the reaction of OH radicals with guanine residue, using an immunofluorescence staining for 1) mouse brain tissue, 2) human A549 cells, and 3) DNA thin film. Positive stains were evident particularly in the peripheral region of the cerebrum and in Purkinje cells of the cerebellum in mice. The immunofluorescence staining of cell nuclei was detected at a dose as small as 5 Gy where we can distinguish each incoming particle. DNA film with an insoluble property to water was developed with special reference to the uniformity, and the treatment with Fenton reaction to generate OH radicals resulted in the successful staining of uniformly formed 8-OHdG.

为了可视化半影（离子轨迹周围的低 LET 区域）的分布，我们开发了使用免疫荧光染色对 8-OHdG 的分布进行成像的方法，8-OHdG 是 OH 自由基与鸟嘌呤残基反应产生的辐射产物。 1) 小鼠脑组织，2) 人类 A549 细胞，3) DNA 薄膜。阳性染色在小鼠大脑的周边区域和小脑的浦肯野细胞中尤其明显。在小至 5 Gy 的剂量下检测细胞核的免疫荧光染色，我们可以区分每个进入的颗粒。特别注重均匀性，开发了不溶于水的DNA薄膜，并通过Fenton反应处理产生OH自由基，成功对均匀形成的8-OHdG进行染色。

项目成果

Role of wild type p53 in apoptotic and non-apoptotic cell death induced by X-irradiation and heat treatment in p53-mutated mouse M10 cells

野生型 p53 在 p53 突变小鼠 M10 细胞 X 辐射和热处理诱​​导的凋亡和非凋亡细胞死亡中的作用

• 发表时间: 2010
• 期刊: Journal of Radiation Research
• 影响因子: 2
• 作者: A.Ito;et al.
• 通讯作者: et al.

トラック構造と生物効果

追踪结构和生物效应

• 发表时间: 2011
• 作者: Ryoichi Hirayama;Yoshitaka Matsumoto;Akiko Uzawa;Nobuhiro Takase;Chizuru Tsuruoka;Mami Wada;Miho Noguchi;Yuki Kase;Naruhiro Matsufuji;Atsushi Ito;Ryuichi Okayasu;Yoshiya Furusawa;平山亮一;平山亮一
• 通讯作者: 平山亮一

Mitochondrial reactive oxygen species generation by the SDHC V69E mutation causes low birth weight and neonatal growth retardation

• DOI: 10.1016/j.mito.2010.09.006
• 发表时间: 2011-01-01
• 期刊: MITOCHONDRION
• 影响因子: 4.4
• 作者: Ishii, Takamasa;Miyazawa, Masaki;Ishii, Naoaki
• 通讯作者: Ishii, Naoaki

Direct and indirect actions to high-LET radiations

对高 LET 辐射的直接和间接作用

• 发表时间: 2010
• 作者: Ryoichi Hirayama;Yoshitaka Matsumoto;Yuki Kase;Miho Noguchi;Nobuhiro Takase;Akiko Uzawa;Atsushi Ito;Naruhiro Matsufuji;Koichi Ando;Ryuichi Okayasu;Yoshiya Furusawa
• 通讯作者: Yoshiya Furusawa

athematical models of the generation of radiation-induced DNA double-strand breaks

辐射诱导 DNA 双链断裂产生的数学模型

• 发表时间: 2012
• 期刊: J. Mathematical Biol
• 作者: 赤松剛;他6名;Y. Saisho and A. Ito
• 通讯作者: Y. Saisho and A. Ito