Establishment of a novel method for retinal cell differentiation using human cells derived from child eyeballs

建立利用儿童眼球来源的人类细胞分化视网膜细胞的新方法

• 批准号: 22591975
• 负责人: NISHINA Sachiko
• 金额: $ 2.91万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591975/
• 关键词: 視覚障害; 先天性疾患; 再生医療; ES細胞; 網膜芽細胞種; 低分子量化合物; p38MAP; キナーゼ; SB203580; 網膜芽細胞腫; p38MAPキナーゼ

We aimed to identify small molecules that can induce neuronal cell differentiation. As the results, we show that 1) the p38 MAPK-specific inhibitor SB203580 blocks the spontaneous differentiation of ES cells into cardiomyocytes, and instead induces the differentiation of these ES cells into neurons 2) p38 regulates BMP2 gene expression through the transcription factor MEF2C, and 3) JNK signaling pathway regulates axon elongation. Furthermore, 4) we screened 1,600 well-characterized small molecule compounds and identified about 20 drugs that stimulate neuronal differentiation.

我们旨在鉴定可以诱导神经元细胞分化的小分子。 As the results, we show that 1) the p38 MAPK-specific inhibitor SB203580 blocks the spontaneous differentiation of ES cells into cardiomyocytes, and instead induces the differentiation of these ES cells into neurons 2) p38 regulates BMP2 gene expression through the transcription factor MEF2C, and 3) JNK signaling pathway regulates axon elongation.此外，4）我们筛选了1,600个特征良好的小分子化合物，并鉴定出约20种刺激神经元分化的药物。

项目成果

Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex

• DOI: 10.1523/jneurosci.1111-11.2011
• 发表时间: 2011-09
• 期刊: Neuroscience Research
• 影响因子: 2.9
• 作者: Tokiwa Yamasaki;H. Kawasaki;H. Nishina

乳幼児の眼鏡処方

婴儿配镜处方

• 发表时间: 2012
• 作者: Tsutsumi N;Tomikawa M;Uemura M;Akahoshi T;Nagao Y;Konishi K;Ieiri S;Hong J;Maehara Y;Hashizume M;Ueta M;仁科幸子
• 通讯作者: 仁科幸子

A case with stereopsis following new surgical procedure for large-angle esotropia in Duane syndrome

杜安综合征大角度内斜视新手术后立体视觉一例

• 发表时间: 2012
• 作者: Ieiri S;Ohdaira T;Tomikawa M;Hashizume M;Taguchi T;et.al;Ueta M;Nishina S et al.
• 通讯作者: Nishina S et al.

小眼球症に伴う眼窩発育異常の画像評価

与小眼症相关的眼眶发育异常的图像评估

• 发表时间: 2012
• 期刊: 眼臨紀
• 作者: 仁科幸子;近藤寛之
• 通讯作者: 近藤寛之

Two novel mutations in the EYS gene are possible major causes of autosomal recessive retinitis pigmentosa in the Japanese population.

• DOI: 10.1371/journal.pone.0031036
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Hosono K;Ishigami C;Takahashi M;Park DH;Hirami Y;Nakanishi H;Ueno S;Yokoi T;Hikoya A;Fujita T;Zhao Y;Nishina S;Shin JP;Kim IT;Yamamoto S;Azuma N;Terasaki H;Sato M;Kondo M;Minoshima S;Hotta Y
• 通讯作者: Hotta Y