Mechanism of leucine signaling of Thermus thermophilus
嗜热栖热菌亮氨酸信号传导机制
基本信息
- 批准号:22780084
- 负责人:
- 金额:$ 2.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2010
- 资助国家:日本
- 起止时间:2010 至 2011
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We revealed that glutamate dehydrogenase(GDH) from Thermus thermophilus formed hetero-hexameric structure consisted from homologous two subunits ; catalytic subunit(GdhB) and regulatory subunit(GdhB), and this complex was subject to allosteric activation by hydrophobic amino acids especially leucine. We succeeded to determine the crystal structure of GdhA/GdhB/Leu complex and revealed the allosteric mechanism of GDH. GDH2 from human(hGDH2) is also subject to allosteric activation by leucine and we found that important residues for binding of leucine were conserved in hGDH2. The mutational analyses of hGDH2 revealed that hGDH2 was also allosterically activated by leucine with a similar manner with GDH from T. thermophilus.We also performed comprehensive analysis of gene regulation by means of DNA microarray tecnique and suggested that leucine was a signal of global regulation in T. thermophilus.
我们发现来自嗜热栖热菌的谷氨酸脱氢酶(GDH)形成由同源的两个亚基组成的异六聚体结构;该复合物由催化亚基(GdhB)和调节亚基(GdhB)组成,并且该复合物受到疏水性氨基酸特别是亮氨酸的变构激活。我们成功确定了GdhA/GdhB/Leu复合物的晶体结构,揭示了GDH的变构机制。来自人类的GDH2(hGDH2)也受到亮氨酸的变构激活,我们发现用于结合亮氨酸的重要残基在hGDH2中是保守的。 hGDH2的突变分析表明,hGDH2也被亮氨酸变构激活,其方式与嗜热链球菌GDH相似。我们还利用DNA微阵列技术对基因调控进行了全面分析,表明亮氨酸是体内全局调控的信号。嗜热链球菌。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Mulecular mechanism for allosteric regulation of glutamatedehydrogenase from Thermus thermophilus
嗜热栖热菌谷氨酸脱氢酶变构调节的分子机制
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:富田武郎;西山真
- 通讯作者:西山真
Thermus thermophilus由来グルタミン酸脱水素酵素における活性調節の分子機構の解析
嗜热栖热菌谷氨酸脱氢酶活性调控的分子机制分析
- DOI:
- 发表时间:2010
- 期刊:
- 影响因子:0
- 作者:富田武郎;西山真
- 通讯作者:西山真
Mechanism of leucine-induced allosteric activation of glutamate dehydrogenase
亮氨酸诱导谷氨酸脱氢酶变构激活的机制
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:富田武郎;西山真
- 通讯作者:西山真
高度好熱菌Thermus thermophilus由来グルタミン酸脱水素酵素のロイシンによるアロステリック調節の構造基盤
亮氨酸对嗜热栖热菌谷氨酸脱氢酶变构调节的结构基础
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:富田武郎;葛山智久;西山真
- 通讯作者:西山真
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TOMITA Takeo其他文献
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{{ truncateString('TOMITA Takeo', 18)}}的其他基金
Functional analysis and molecular basis of leucine as a biological regulator
亮氨酸作为生物调节剂的功能分析和分子基础
- 批准号:
24580137 - 财政年份:2012
- 资助金额:
$ 2.58万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of regulatory mechanism of aspartate kinase
天冬氨酸激酶调控机制分析
- 批准号:
19780054 - 财政年份:2007
- 资助金额:
$ 2.58万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
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