The role of transcription factor Tead1 in the differentiation and proliferation of retinal pigment epithelial cells.

转录因子Tead1在视网膜色素上皮细胞分化和增殖中的作用

• 批准号: 22791669
• 负责人: MICHINORI Kitagawa
• 金额: $ 1.58万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791669/
• 关键词: 眼発生; 再生医学; 細胞増殖; 転写因子; 網膜色素上皮細胞; ES細胞; 細胞分化

I studied the role of transcription factor Tead1 in the differentiation and proliferation of retinal pigment epithelial cells by using a mutant of Tead1 identified in human Sveinsson' s chorioretinal atrophy. Tead1 or its mutant was expressed in mouse embryonic primary retinal pigment epithelial cells culture, and the cells were analyzed for the proliferation and the expression of Tead target genes. In addition, I clarified the expression patterns of Tead transcription factors and the coactivators, YAP and TAZ, during eye development in mouse embryos.

我通过使用在人类Sveinsson的脉络膜视网膜萎缩中鉴定出的TEAD1突变体来研究转录因子TEAD1在视网膜色素上皮细胞分化和增殖中的作用。 TEAD1或其突变体在小鼠胚胎原发性视网膜色素上皮细胞培养中表达，并分析细胞的增殖和表达靶基因。此外，我在小鼠胚胎的眼睛发育过程中阐明了TEAD转录因子和YAP和TAZ的表达模式。

项目成果

Differentiation of mesodermal cells from pluripotent stem cells

• DOI: 10.1007/s12185-010-0518-8
• 发表时间: 2010-04-01
• 期刊: INTERNATIONAL JOURNAL OF HEMATOLOGY
• 影响因子: 2.1
• 作者: Kitagawa, Michinori;Era, Takumi
• 通讯作者: Era, Takumi