Analysis of the role of androgen receptor in granulosa cells during the oocyte maturation.

卵母细胞成熟过程中颗粒细胞雄激素受体的作用分析。

基本信息

批准号：
22791556
负责人：
HAYASHI Atsushi
金额：
$ 2.16万
依托单位：
Osaka Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2010
资助国家：
日本
起止时间：
2010 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791556/
关键词：
不妊症アンドロゲンレセプター子宮内膜症

项目摘要

BACKGROUND : Endometriosis is one of the most common gynecological diseases and is frequently associated with infertility. Endometriosis may affect the androgen receptor(AR) mRNA expression in human granulosa cells and the methylation of the promoter region of AR. We investigated 28 patients with endometriosis and 47 subjects without endometriosis undertaking IVF treatment. METHODS : Granulosa cells were obtained from 28 patients with endometriosis and 47 subjects without endometriosis as a control, then expression of AR and FSHR mRNA were evaluated by StepOne real-time PCR analysis. The methylation of the promoter region was qualified by methylation-specified PCR(MSP). RESULTS : The expression of AR mRNA in endometriosis was statistically lower than control and FSHR mRNA expression in control showed a positive correlation with AR mRNA expression ; however, there was no such correlation in endometriosis patients. In the control, AR mRNA expression was statistically higher in pregnant subjects compared with non-pregnant subjects ; however, in endometriosis, no significant difference was identified. The promoter of AR was heavily methylated in all cases of endometriosis ; however, only 5(45.4%) were methylated in the control. CONCLUSION : Lower AR mRNA expression and methylation of the AR promoter region might affect the expression of AR and FSHR in endometriosis, leading to a disturbance of the regulation of AR and FSHR.

背景：子宫内膜异位症是最常见的妇科疾病之一，常常与不孕症相关。子宫内膜异位症可能影响人颗粒细胞中雄激素受体（AR）mRNA的表达以及AR启动子区的甲基化。我们调查了 28 名子宫内膜异位症患者和 47 名接受 IVF 治疗的无子宫内膜异位症受试者。方法：取28例子宫内膜异位症患者和47例无子宫内膜异位症患者的颗粒细胞作为对照，通过StepOne实时PCR分析评估AR和FSHR mRNA的表达。通过甲基化特异性PCR（MSP）对启动子区域的甲基化进行鉴定。结果：子宫内膜异位症组AR mRNA表达量明显低于对照组，对照组FSHR mRNA表达量与AR mRNA表达量呈正相关；然而，子宫内膜异位症患者却没有这种相关性。在对照组中，与非怀孕受试者相比，怀孕受试者的 AR mRNA 表达在统计学上更高；然而，在子宫内膜异位症中，没有发现显着差异。在所有子宫内膜异位症病例中，AR 启动子均高度甲基化；然而，对照中只有 5 个（45.4%）被甲基化。结论:AR mRNA表达降低和AR启动子区甲基化可能影响子宫内膜异位症中AR和FSHR的表达,导致AR和FSHR的调节紊乱。