Detection of free intraperitoneal cancer cells and chemosensitivity testing using gene expression analysis for gastric cancer patients

胃癌患者腹膜内游离癌细胞的检测及基因表达分析的化疗敏感性试验

• 批准号: 22591473
• 负责人: ITO Seiji
• 金额: $ 3.08万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591473/
• 关键词: 癌; 微小転移; 抗癌剤受性; 抗癌剤感受性; 胃がん

This study aimed at integrated development of the genetic detection of peritoneal micrometastases and effective therapy based on biological characteristics of peritoneal micrometastases. Microarray analysis was performed for the cell lines with low CEA expression and new marker genes were identified. The predicting model for peritoneal metastasis was constructed. Both sensitive and resistant cell lines for paclitaxel were establishedandgene expression profiling was compared with parent cell line. The selected genes were validated by clinical samples andidentified as paclitaxel-resistance related genes.

本研究旨在综合开发腹膜微转移的基因检测和基于腹膜微转移生物学特性的有效治疗。对 CEA 表达低的细胞系进行微阵列分析，并鉴定出新的标记基因。构建腹膜转移预测模型。建立了对紫杉醇敏感和耐药的细胞系，并与亲本细胞系进行基因表达谱比较。所选基因经临床样本验证，鉴定为紫杉醇耐药相关基因。

项目成果

胃癌腹膜再発予測モデルを用いたmultiple genetic markerの意義の検討

使用胃癌腹膜复发预测模型检查多种遗传标记的意义

• 发表时间: 2010
• 作者: Fischera A;Zoccali M;Kono T;伊藤誠二
• 通讯作者: 伊藤誠二

Comprehensive analysis of predictive markers for paclitaxel-resistance against peritoneal metastasis of gastric cancer

胃癌腹膜转移紫杉醇耐药预测标志物综合分析

• 发表时间: 2011
• 作者: 西毅;小林直木;久野悠;山口明人;Murakami H
• 通讯作者: Murakami H

The prognostic significance of isolated tumor cells in the lymph nodes of gastric cancer patients

• DOI: 10.1007/s10120-010-0556-1
• 发表时间: 2010-08-01
• 期刊: GASTRIC CANCER
• 影响因子: 7.4
• 作者: Fukagawa, Takeo;Sasako, Mitsuru;Shimoda, Tadakazu
• 通讯作者: Shimoda, Tadakazu

"In Vivo Imaging" Methods and Protocols

“体内成像”方法和方案

• 发表时间: 2010
• 作者: Nakanishi;H;Ito S
• 通讯作者: Ito S

Development of rapid isolation device for circulating tumor cells (CTCs) using size-based filtrationmethod and its application for single cell gene expression analysis

基于尺寸的过滤方法开发循环肿瘤细胞（CTC）快速分离装置及其在单细胞基因表达分析中的应用

• 发表时间: 2012
• 作者: YusaA,MasudaT;Yamamoto S;Niimi M;Douke H;Okochi M;Toneri M;Ito S;Honda H;Arai F;Nakanishi H.
• 通讯作者: Nakanishi H.