Development of Early Detection kit for diagnosis mucormycosis

毛霉菌病早期检测试剂盒的开发

• 批准号: 22591110
• 负责人: KAKEYA Hirosi
• 金额: $ 3万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591110/
• 关键词: 接着菌; シグナルシークエンス; 診断キット; 接合菌

The diagnosis of mucormycosis relies upon the identification of organisms in tissue by histopathology with culture confirmation. However, culture often yields no growth, and histopathologic identification of an organism with a structure typical of Mucorales may provide the only evidence of infection. A clinician must think of this entity in the appropriate clinical setting and pursue invasive testing in order to establish a diagnosis as early as possible. Therefore, new early detection methods are required. We used a novel signal sequence trap methods to select protein antigen from Rhizopus oryzae. Two unknown proteins with signal sequences, antigen A (26kDa, hypothetical protein) and B (46kDa, hypothetical protein) were selected as candidates. We made ELISA kit to detect antigen A. The antigen A was detected in the culture supernatant of R. oryzae、R microspores、R microspores var. rhizopodiformis. The antigen was also detected in the serum form Rhizopus infected mice.

通过培养的组织病理学对组织中的生物鉴定，粘液菌病的诊断依赖于培养物的生长。在适当的临床环境中，我们需要在办公室进行检测方法。 B（46KDA，假设的蛋白质）作为糖果。形成根茎的感染小鼠。

项目成果

Clinical efficacy and safety micafungin in Japanese patients with chronic pulmonary aspergillosis: a prospective observational study

米卡芬净治疗日本慢性肺曲霉病患者的临床疗效和安全性：一项前瞻性观察研究

• 发表时间: 2012
• 期刊: Med Mycol.
• 作者: Kohno S;Izumikawa K;Kakeya H;Miyazaki Y;Ogawa K;Amitani R;Niki Y;Kurashima A
• 通讯作者: Kurashima A

Multilocus sequence typing of Cryptococcus neoformans in non-HIV associated cryptococcosis in Nagasaki, Japan

• DOI: 10.3109/13693786.2012.708883
• 发表时间: 2013-04-01
• 期刊: MEDICAL MYCOLOGY
• 影响因子: 2.9
• 作者: Mihara, Tomo;Izumikawa, Koichi;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

Diagnostic significance of Aspergillus species isolated from respiratory samples in an adult pneumology ward

• DOI: 10.3109/13693786.2010.548084
• 发表时间: 2011-08-01
• 期刊: MEDICAL MYCOLOGY
• 影响因子: 2.9
• 作者: Tashiro, Takayoshi;Izumikawa, Koichi;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

Functional characterization of the regulators of calcineurin in Candida glabrata

• DOI: 10.1111/j.1567-1364.2011.00751.x
• 发表时间: 2011-12-01
• 期刊: FEMS YEAST RESEARCH
• 影响因子: 3.2
• 作者: Miyazaki, Taiga;Izumikawa, Koichi;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

Skn7p is required for oxidative stress response and virulence in Candida glabrata

Skn7p 是光滑念珠菌氧化应激反应和毒力所必需的

• 发表时间: 2010
• 期刊: Mycopathologia
• 影响因子: 5.5
• 作者: Saijo T;Miyazaki T;Izumikawa K;Mihara T;Takazono T;Kosai K;Imamura Y;Seki M;Kakeya H;Yamamoto Y;Yanagihara K;Kohno S
• 通讯作者: Kohno S