Molecular characterization of Marek's disease virus genomes isolated from white-fronted geese

从白额雁中分离的马立克氏病病毒基因组的分子特征

• 批准号: 15405038
• 负责人: OHASHI Kazuhiko
• 金额: $ 7.81万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15405038/
• 关键词: Marek's disease virus; white-fronted goose; nested PCR; ヒシクイ; meq遺伝子

To characterize the genomes of Marek's disease virus serotype 1 (MDV 1) isolated from wild geese, feather tips and blood samples were collected from 200 pintails in Hokkaido, 63 bean geese (Anser fabalis), 15 canada geese (Branta canadensis), and 50 white-fronted geese (Anser Albifrons) in the fareast area of Russia during 2003-2005. Samples were also collected from 147 white-fronted geese at Hokkaido during 2003-2005. Total cellular DNAs were extracted from these samples, and the meq gene-specific nested PCR was performed to detect the MDV 1 genome. The MDV genome was not detected from pintails, but 25-30% of samples from thses geese were positive for the meq gene. These results showed that MDV 1 is present in wild goose populations though no clinical signs were observed in these geese.In the next experiment, nucleotide sequence analysis of the MDV1 genomes obtained from white-fronted geese was done to estimate the pathogenicities of the MDV1 strains. The nucleotide sequences of the meq genes of the MDV1 strains were very similar to those of very virulent MDV1 reported previously. In addition, a deletion in the glycoprotein (g) L gene, which was reported in the very virulent MDV1, was also found in the MDV1 strains from white-fronted geese. These results suggested that the MDV1 strains detected in white-fronted geese are very virulent. However, nucleotide sequence of the gB gene of goose-derived MDV1 was slightly different from that of very virulent MDV1 isolated from chickens in the Hokkaido area, showing that the MDV1 transmission between chickens and geese is not so frequent at the present time. It should be noted that the meq gene of one MDV1 strain was very similar to that of very virulent MDV1 isolated from chickens in the Hokkaido area, and it will be necessary to study larger numbers of samples from goose populations.

为了表征从野雁中分离出的马立克氏病病毒血清型 1 (MDV 1) 的基因组，我们从北海道的 200 只针尾雁、63 只豆雁 (Anser fabalis)、15 只加拿大雁 (Branta canadensis) 和 50 只雁中采集了羽尖和血液样本。俄罗斯远东地区的白额雁（Anser Albifrons） 2003-2005。 2003 年至 2005 年期间，我们还从北海道的 147 只白额雁身上采集了样本。从这些样本中提取总细胞 DNA，并进行 meq 基因特异性巢式 PCR 来检测 MDV 1 基因组。针尾鹅中未检测到 MDV 基因组，但这些鹅的样本中 25-30% 的 meq 基因呈阳性。这些结果表明MDV 1存在于雁群中，尽管在这些雁中没有观察到临床症状。在接下来的实验中，对从白额雁获得的MDV1基因组进行了核苷酸序列分析，以估计MDV1毒株的致病性。 MDV1毒株的meq基因的核苷酸序列与之前报道的强毒力MDV1的核苷酸序列非常相似。此外，在白额雁的 MDV1 毒株中也发现了高毒力 MDV1 中报道的糖蛋白 (g) L 基因的缺失。这些结果表明，在白额雁中检测到的 MDV1 毒株具有很强的毒性。然而，鹅源性MDV1的gB基因的核苷酸序列与从北海道地区鸡中分离出的强毒力MDV1的核苷酸序列略有不同，这表明目前鸡和鹅之间的MDV1传播并不那么频繁。值得注意的是，一种 MDV1 毒株的 meq 基因与从北海道地区鸡中分离出的高毒力 MDV1 的 meq 基因非常相似，因此有必要研究来自鹅群的大量样本。

项目成果

マレック病ウイルスによる潜伏感染・腫瘍化におけるL-meq遺伝子の役割

L-meq基因在马立克氏病病毒潜伏感染和肿瘤发生中的作用

• 发表时间: 2004
• 作者: 大橋和彦ら

Identification and characterization of peptides binding to Newcastle disease virus by phage display.

通过噬菌体展示鉴定和表征与新城疫病毒结合的肽。

• 发表时间: 2005
• 作者: Ozawa; M.; et al.
• 通讯作者: et al.

Loop-Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus

环介导等温扩增快速检测新城疫病毒

• DOI: 10.1128/jcm.43.4.1646-1650.2005
• 发表时间: 2005-04-01
• 期刊: Journal of Clinical Microbiology
• 影响因子: 9.4
• 作者: H. M. Pham;C. Nakajima;K. Ohashi;M. Onuma
• 通讯作者: M. Onuma

Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1.

检测感染马立克氏病病毒血清型 1 的鸡 T 细胞亚群中 meq 基因。

• 发表时间: 2005
• 作者: Chang; K.S.; et al.
• 通讯作者: et al.

Molecular characterization of the nucleocapsid protein gene of Newcastle disease virus strains in Japan and development of a restriction enzyme-based rapid identifying method.

日本新城疫病毒株核衣壳蛋白基因的分子特征及基于限制性内切酶的快速鉴定方法的开发。

• 发表时间: 2004
• 作者: Pham; H.M.; et al.
• 通讯作者: et al.