Integrated analyses of virulence factors from Staphylococcus aureusaiming to pursue novel pathogenic mechanisms and chemotherapy based on new concept

金黄色葡萄球菌毒力因子综合分析，寻求新的致病机制及新理念化疗

• 批准号: 22590402
• 负责人: BABA Tadashi
• 金额: $ 2.83万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590402/
• 关键词: 黄色ブドウ球菌; 赤血球溶解毒素; β-ヘモリジン; ヒトケラチノサイト; アトピー性皮膚炎; nybomycin; キノロン; gyrase; ヒト皮膚角化細胞; バクテリオファージ; 病原性; ケラチノサイト; 病原性アイランド; 細胞毒性; 免疫細胞; 宿主特異性; MHC; TSLP; 好中球

On the way to pursue novel pathogenic mechanisms that can be candidates for targets of novel chemotherapy, I found ・-hemolysin, known as an erythrolytic toxin from Staphylococcus aureus, damages human keratinocytes. This result suggests that the toxin is presumably involved in atopic dermatitis that is worsened by S. aureus infection, and the finding may lead to further understanding of the disease and its therapy. I also played part in research on re-evaluation of nybomycin, that had been isolated from actinobacteria over 50 years ago, and showed that the substance specifically inhibits function of mutated bacterial DNA gyrase that was no longer inactivated by fluoroquinolones, whereas nybomycin-resistant DNA gyrase acquired fluoroquinolone susceptibility, indicating fluoroquinolones and nybomicin have mutually complementary functions. Such substance against known antibiotic was designated ‘reverse antibiotic’, being expected to open new era of antibiotic chemotherapy. It should be noted that the results described above were obtained cooperative researches with scientists who were originally out of this project.

在追求新型致病机制的途径上，这些机制可以成为新型化学疗法靶标的候选者，我发现・-蛋白酶蛋白，被称为金黄色葡萄球菌的红细胞素化毒素，会损害人角质形成细胞。该结果表明，毒素可能参与了金黄色葡萄球菌感染恶化的特应性皮炎，这一发现可能会导致对疾病及其疗法的进一步了解。 I also played part in research on re-evaluation of nybomycin, that had been isolated from actinobacteria over 50 years ago, and showed that the substance specifically inhibits function of mutualized bacteria DNA gyrase that was no longer inactivated by fluoroquinolonones, whereas nybomycin-resistant DNA gyrase acquired fluoroquinolonones susceptibility, indicating氟喹诺酮类和氯酸酯具有相互互补的功能。这种针对已知抗生素的物质被指定为“反向抗生素”，预计将开放抗生素化疗的新时代。应当指出，上述结果是与最初不在该项目之外的科学家获得的合作研究。

项目成果

MHC analogues found in S.aureus affect phagocytosis and other immune responses

金黄色葡萄球菌中发现的 MHC 类似物影响吞噬作用和其他免疫反应

• 发表时间: 2012
• 作者: M.Sekine;T.Baba;Y.Katayama;K.Hiramatsu
• 通讯作者: K.Hiramatsu

黄色ブドウ球菌MHC様分子が貪食などの宿主免疫系に及ぼす影響について(MHC analogues found in Staphylococcus aureus affect phagocytosis and other immune responses by human whit e-blood cells)

金黄色葡萄球菌中发现的 MHC 类似物影响人类白细胞的吞噬作用和其他免疫反应

• 发表时间: 2011
• 期刊: 順天堂医学
• 作者: 関根美和;他
• 通讯作者: 他

Expression and Functional Characterization of Retinoic Acid-Inducible Gene-I-Like Receptors of Mast Cells in Response to Viral Infection

• DOI: 10.1159/000343895
• 发表时间: 2013-01-01
• 期刊: JOURNAL OF INNATE IMMUNITY
• 影响因子: 5.3
• 作者: Fukuda, Minoru;Ushio, Hiroko;Ogawa, Hideoki
• 通讯作者: Ogawa, Hideoki

新規化合物、DNAジャイレース阻害剤及びその用途

新化合物、DNA旋转酶抑制剂及其用途

• 发表时间: 2013

毒素検出方法

毒素检测方法

• 发表时间: 2012