Comparative genetical analysis among South American indigenous populations

南美土著人群的比较遗传分析

• 批准号: 22570231
• 负责人: DANJO Inaho
• 金额: $ 3万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570231/
• 关键词: 南米; 先住民族; 少数民族; B-LCL; B細胞株; ゲノム解析; マイクロサテライト; アレイCGH; リソース

We established B lymphoblastoid cell lines (EBV-B cell) from peripheral blood mononuclear cell stocks obtained from 550 South American indigenous individuals. We performed analysis of genetical relationships among tribes to make clear the migration rout of mongoloid populations from Eurasia to South America. Before starting genetical analysis, we checked the genomic stability of EBV-B cells during establishment and long-term culture periods, and showed that the cells retain stable genome structures by genetical studies. For the genetical analysis, samples were divided into 5 groups according to the geographical location, and then figured out the genetical features with forensic microsatellite markers. The results showed that each group retained smaller heterogeneity and specific allele frequency patterns for each microsatellite locus. These results suggest that Amerind populations are not so homogenous as a whole but can be divided into some genetically-diverse groups.

我们从550个南美本土个体获得的外周血单核细胞库存中建立了B淋巴母细胞系（EBV-B细胞）。我们对部落之间的遗传关系进行了分析，以清楚蒙古人种群从欧亚大陆到南美的迁移。在开始基因分析之前，我们检查了在建立和长期培养期间EBV-B细胞的基因组稳定性，并表明细胞通过遗传研究保留了稳定的基因组结构。对于基因分析，根据地理位置将样品分为5组，然后用法医微卫星标记弄清楚了遗传特征。结果表明，每个小组为每个微卫星基因座保留了较小的异质性和特定等位基因频率模式。这些结果表明，Amerind人群的整体不是那么同质，但可以分为某些遗传多样性群体。

项目成果

Genetic relationship among ethnic minority groups in South America from the viewpoint of autosomal and Y chromosomal STRs

从常染色体和Y染色体STR角度看南美洲少数民族的遗传关系

• 发表时间: 2011
• 作者: Yamamoto;T.;Ito;T.;Gomyoda;T.;Saijo;K.;Danjoh;I.;Nakamura;Y.
• 通讯作者: Y.

理研細胞バンクにおける細胞の品質管理と標準化

RIKEN 细胞库的细胞质量控制和标准化

• 发表时间: 2010
• 期刊: Tissue Culture Research Communications
• 作者: 檀上稲穂;中村幸夫
• 通讯作者: 中村幸夫

がん細胞株・不死化細胞を用いた研究, 目的別で選べる細胞培養プロトコール、中村幸夫編

使用癌细胞系和永生化细胞的研究，可根据目的选择的细胞培养方案，中村幸雄编辑

• 发表时间: 2012
• 作者: Noguchi-Kato;Hisashi;Kenji Nitta and Tomio Itani;保田謙太郎・青木大輔・中山祐一郎・山口裕文;檀上稲穂
• 通讯作者: 檀上稲穂

Collection of peripheral blood mononuclear cells obtained from Amerindians and other Mongoloid minority groups.

采集自美洲印第安人和其他蒙古人种少数民族的外周血单核细胞。

• 发表时间: 2010
• 作者: Inaho Danjoh;Kaoru Saijo;Kaoru Fukami-Kobayashi;Yukio Nakamura
• 通讯作者: Yukio Nakamura

Collection of peripheral blood mononuclear cells obtained from Amerindians and other Mongoloid minority groups

采集自美洲印第安人和其他蒙古人种少数民族的外周血单核细胞

• 发表时间: 2010
• 作者: I.Danjoh;K.Saijo;K.Fukami-Kobayashi;Y.Nakamura
• 通讯作者: Y.Nakamura