Maintenance of bone mineral density in Senescence Accelerated MiceP6 (SAMP6)

衰老加速小鼠 P6 (SAMP6) 中骨矿物质密度的维持

• 批准号: 22500684
• 负责人: KATSUYAMA Hironobu
• 金额: $ 2.75万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500684/
• 关键词: 加齢; 老化; 老化促進マウス; ビタミンK2; 骨形態計測; 水浸拘束ストレス; 骨代謝; 骨代謝マーカー; 生理活性物質

The P6 strain of senescence-accelerated mice (SAMP6) exhibit features of skeletal aging including reduced bone mineral density (BMD), so that various physiological active agents affecting BMD could be examined. We developed the further bone resorption model using water-immersion restraint stress (WRS). Male SAMP6 were divided into three groups, i.e., control group, WRS (6 h/day, 5 times/wk) group, and WRS + vitamin K2 (VK2) (30 mg/kg s.c.) group. SAMP6 were suffered from this stress between 6 to 10 weeks old. BMD was measured using computed tomography (CT) system for small animals, and fluctuation of bone turnover markers and bone histomorphometrical analysis were also performed.BMD of trabecular bone for WRS groups was significantly lower than that of control group, suggesting that WRS was effective to reduce BMD. However, no significant difference of BMD was observed between WRS group and WRS + VK2 group. In bone turnover markers, urinary excretion of CTX, bone resorption mark，was significantly higher in WRS groups than in control. And urinary excretionof calcium was significantly higher in WRS group than in other groups. On the other hand, bone histomorphometrical analysis indicated that trabeculae of WRS showed sthin and mall shape, but that of WRS + VK2 showed larger and round shape. On the resorption area, large osteoclast was observed in WRS, but the number of osteoclasts was significantly decreased in WRS + VK2. These resultsindicated that vitamin K2 could affect bone turnover.

骨骼老化的P6菌株，包括降低的骨矿物质密度（BMD），因此可以检查各种影响BMD的物理活性剂。我们使用水免疫约束应力（WRS）开发了进一步的骨骼分辨率模型。将男性SAMP6分为三组，即对照组，WRS（6 h/天，5次/周），WRS +维生素K2（VK2）（30 mg/kg S.C.）组。 SAMP6在6至10周大的情况下遭受了这种压力。还使用计算机断层扫描（CT）系统来测量BMD，并进行骨转换标记和骨骼组织形态计量分析的波动。WRS组的小梁骨的BMD明显低于对照组的BMD，这表明WRS有效减少BMD。但是，WRS组和WRS + VK2组之间没有观察到BMD的显着差异。在骨转换标记中，CTX的尿极端，骨骼分辨率标记在WRS组中明显高于对照组。 WRS组的尿液极端极端异常明显高于其他组。另一方面，骨骼组态计量学分析表明，WR的小梁表现出STHIN和购物中心的形状，但WRS + VK2的形状显示大而圆形。在分辨率区域，在WRS中观察到大破骨细胞，但是在WRS + VK2中，破骨细胞的数量显着减少。这些结果表明，维生素K2可能影响骨骼更新。

项目成果

老化促進マウスを用いた骨密度減少モデルの作成

使用加速衰老的小鼠创建骨密度降低模型

• 发表时间: 2012
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信;勝山博信;勝山博信
• 通讯作者: 勝山博信

Vitamin K2 administration and bone mineral density in Senescence Accelerated Mice P-6 (SAMP6)

维生素 K2 给药与衰老加速小鼠 P-6 (SAMP6) 的骨矿物质密度

• 发表时间: 2012
• 作者: Hironobu Katsuyama;Shigeko Fushimi;Kunikazu Yamane;Hajime Hinenoya;Yuji Akiyama;Masafumi Tomita;Toshiko Okuyama;Yoko Watanabe;Midori Katsuyama;Le Ngoc Anh;Kiyofumi Saijoh
• 通讯作者: Kiyofumi Saijoh

マウス骨密度に及ぼす水浸拘束ストレスの影響

水浸约束应力对小鼠骨密度的影响

• 发表时间: 2012
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信;勝山博信
• 通讯作者: 勝山博信

ビタミンK2がストレスモデルマウスの骨形態におよぼす影響

维生素K2对应激模型小鼠骨形态的影响

• 发表时间: 2013
• 作者: 伏見滋子;富田正文;奥山敏子;渡辺洋子;山根一和;日根野谷一;秋山祐治;西條清史;勝山碧;勝山博信
• 通讯作者: 勝山博信

Water-restraint stress enhances methamphetamine-induced cardiotoxicity.

• DOI: 10.1016/j.cbi.2011.01.025
• 发表时间: 2011-03
• 期刊: Chemico-biological interactions
• 影响因子: 5.1
• 作者: M. Tomita;H. Katsuyama;Yoko Watanabe;Kazuo Hidaka;K. Yoshitome;S. Miyaishi;T. Ishikawa;Kotaro Shinone-Kota