The neuroprotective effect of inhaled hydrogen sulfide against delayed paraplegia after spinal cord ischemia in mice

吸入硫化氢对小鼠脊髓缺血迟发性截瘫的神经保护作用

• 批准号: 22390299
• 负责人: KAKINOHANA Manabu
• 金额: $ 11.4万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390299/
• 关键词: 硫化水素; 遅発性対麻痺; 脊髄虚血; アポトーシス; 神経保護; カスパーゼ; 虚血性脊髄障害; 対麻痺; 一酸化窒素合成酵素; 脊髄運動神経細胞死; 抗炎症効果; 脊髄運動神経細胞

Hydrogen sulfide (H2S), well-known for one of endogenous gas molecules, is reported to possess potency of anti-inflammation, radical scavenging effect,and anti-apoptotic effect, etc. We have investigate the neuroprotective effect of inhaled H2S (80ppm x 5 hrs) against delayed onset paraplegia after spinal cord ischemia in mice. As a result from our experiments, mice treated with inhaled hydrogen sulfide showed significant better neurological outcome at 72 hrs of reperfusion with preserving the number of normal neurons in spinal ventral horn. Additionally, inhaled H2S suppressed the expression of cleaved caspase3 in the spinal ventral horn significantly comparing with control group. According to the present data, it is suggested that inhaled H2S potentially protect spinal ventral neurons after spinal cord ischemia, and this protective effect might be associated with its anti-apoptotic effect.

硫化氢（H2S）是众所周知的内源性气体分子之一，据报道具有抗炎、自由基清除作用和抗细胞凋亡作用等功效。我们研究了吸入H2S（80ppm x 5小时）对抗小鼠脊髓缺血后迟发性截瘫。我们的实验结果表明，吸入硫化氢治疗的小鼠在再灌注 72 小时时表现出明显更好的神经学结果，并且保留了脊髓腹角中正常神经元的数量。此外，与对照组相比，吸入H2S显着抑制脊髓腹角中cleaved caspase3的表达。目前的数据表明，吸入H2S可能对脊髓缺血后的脊髓腹侧神经元有保护作用，这种保护作用可能与其抗凋亡作用有关。