Regulation of virulence expression by retron including reverse transcriptase gene and roles as mobile genetic elements

逆转录子（包括逆转录酶基因）毒力表达的调节及其作为移动遗传元件的作用

• 批准号: 22390083
• 负责人: SHIMAMOTO Tadashi
• 金额: $ 11.9万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390083/
• 关键词: 病原性; 遺伝子発現制御; 逆転写酵素,ゲノム; Vibrio cholerae; レトロン; msDNA; 逆転写酵素; Vibrio; ゲノム; Vibrio choleare

The orf540gene present downstream of the reverse transcriptase gene (ret) in retron-Vc95 was found to be related to pathogenicity in Vibrio choleraeO139. New retrons were isolated and identified from a clinical isolate of V. choleraenon-O1, non-O139 and an environmental isolate of V. mimicus. Plasmid-derived retron of a clinical isolate of Escherichia coliand new retron of Yersinia frederikseniiwere also analysed.

发现反转录酶基因（RET）的ORF540GENE在反vc95中的下游与弧菌霍乱谷中的致病性有关。分离出新的反词，并从V.霍乱的V. Choleraen-O1，非O139和V. mimicus的环境分离株中鉴定出来。还分析了Yersinia yersinia frederikseniiwere的大肠杆菌新回旋的临床分离株的质粒衍生的转子。

项目成果

Genetic analysis of multiple antimicrobial resistance in Salmonella isolated from diseased broilers in Egypt

• DOI: 10.1111/j.1348-0421.2012.00429.x
• 发表时间: 2012-04-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Ahmed, Ashraf M.;Shimamoto, Tadashi
• 通讯作者: Shimamoto, Tadashi

Functional characterization of orf540 of retron-Vc95 in Vibrio cholerae

霍乱弧菌retron-Vc95 orf540的功能表征

• 发表时间: 2011
• 作者: Isomoto H;Moss J;Hirayama T.;Tadashi Shimamoto
• 通讯作者: Tadashi Shimamoto

Complete sequence of a novel multidrug resistance plasmid isolated from a clinical strain of enterohemorrhagic Escherichia coliO157:H7.

从肠出血性大肠杆菌 O157:H7 临床菌株中分离出的新型多药耐药质粒的完整序列。

• 发表时间: 2012
• 作者: Toshi Shimamoto;Mizuki Yamamoto;Ashraf M. Ahmed;Tadashi Shimamoto
• 通讯作者: Tadashi Shimamoto

Vibrio mimicusの逆転写酵素遺伝子を含むレトロンの構造と機能

拟态弧菌逆转录酶基因的结构和功能

• 发表时间: 2012
• 作者: Qu;H.;島本 整,田鳥 安紗子,山下 拓未,神本 真紀,島本 敏
• 通讯作者: 島本 整,田鳥 安紗子,山下 拓未,神本 真紀,島本 敏

Two novel retron elements are replaced with retron-Vc95 in Vibrio cholerae

• DOI: 10.1111/j.1348-0421.2011.00342.x
• 发表时间: 2011-07-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Inouye, Kumiko;Tanimoto, Saori;Shimamoto, Tadashi
• 通讯作者: Shimamoto, Tadashi