Study of MT1-MMP in cancer

MT1-MMP在癌症中的研究

• 批准号: 22220014
• 负责人: SEIKI Motoharu
• 金额: $ 137.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-05-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22220014/
• 关键词: がん; 浸潤・転移; 腫瘍形成; プロテアーゼ; MT1-MMP; コラーゲン; HB-EGF; HIF-1; がん細胞; 浸潤; 増殖; FIH-1; Mint3; リン酸化; 細胞運動; ZF21; p27RF-Rho; HIH-1

Cancer cells are usually destructive against surrounding tissue and this activity is necessary for the cells to continue grow abnormally, invade and form metastatic tumor nodules. Cancer cells also alter the tumor microenvironment by producing different tissue constituents from the normal ones and by changing the integrity of extracellular proteins via protein processing. Degradation and processing of proteins in the tissue space are mediated by extracellular proteases. Membrane type 1 matrix metalloproteinase (MT1-MMP) is one of such extracellular proteases expressed in cancer cells. In this study, we analyzed multidimensional functions of MT1-MMP and their coordinated action with intracellular systems regulating cell growth and invasion. The results suggest that MT1-MMP is important acting as a nordal point regulator during acquisition of malignant phenotype of cancer cells.

癌细胞通常对周围组织具有破坏性，这种活性对于细胞继续异常生长、侵入并形成转移性肿瘤结节是必要的。癌细胞还通过产生与正常组织不同的组织成分以及通过蛋白质加工改变细胞外蛋白质的完整性来改变肿瘤微环境。组织间隙中蛋白质的降解和加工是由细胞外蛋白酶介导的。 1 型膜基质金属蛋白酶 (MT1-MMP) 是癌细胞中表达的细胞外蛋白酶之一。在本研究中，我们分析了 MT1-MMP 的多维功能及其与调节细胞生长和侵袭的细胞内系统的协调作用。结果表明，MT1-MMP 在癌细胞恶性表型的获得过程中作为正常点调节剂发挥着重要作用。

项目成果

Laser scanning-based tissue autofluorescence/fluorescence imaging (LS-TAFI), a new technique for analysis of microanatomy in whole-mount tissues.

基于激光扫描的组织自发荧光/荧光成像（LS-TAFI），一种用于分析整体组织显微解剖的新技术。

• DOI: 10.1016/j.ajpath.2012.02.032
• 发表时间: 2012
• 期刊: The American journal of pathology
• 作者: Mori,Hidetoshi;Borowsky,AlexanderD;Bhat,Ramray;Ghajar,CyrusM;Seiki,Motoharu;Bissell,MinaJ
• 通讯作者: Bissell,MinaJ

Establishment and validation of computational model for MT1-MMP dependent ECM degradation and intervention strategies.

• DOI: 10.1371/journal.pcbi.1002479
• 发表时间: 2012
• 期刊: PLoS computational biology
• 影响因子: 4.3
• 作者: Hoshino D;Koshikawa N;Suzuki T;Quaranta V;Weaver AM;Seiki M;Ichikawa K
• 通讯作者: Ichikawa K

Control and inhibition analysis of complex formation processes.

• DOI: 10.1186/1742-4682-9-33
• 发表时间: 2012-08-03
• 期刊: Theoretical biology & medical modelling
• 作者: Saitou T;Itano K;Hoshino D;Koshikawa N;Seiki M;Ichikawa K;Suzuki T
• 通讯作者: Suzuki T

Ubiquitin ligase RNF126 serves as a gate keeper from glycolysis to oxidative phosphorylation and regulates tumor growth

泛素连接酶 RNF126 作为糖酵解到氧化磷酸化的看门人并调节肿瘤生长

• 发表时间: 2011
• 作者: Seiko Yoshino;Sakamoto T Seiki M.
• 通讯作者: Sakamoto T Seiki M.

新規HIF活性化因子・Mint3の生体における機能の解析

新型 HIF 激活剂 Mint3 的体内功能分析

• 发表时间: 2012
• 作者: 坂本 毅治;原 敏朗;清木 元治
• 通讯作者: 清木 元治