An experimental study on the development of new prevention to the muscle injury for muscle atrophy by using the heat stimulation.

利用热刺激预防肌肉萎缩的肌肉损伤新方法的实验研究。

• 批准号: 21700556
• 负责人: BANNO Yasuhiro
• 金额: $ 2.16万
• 依托单位: Nihon Fukushi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700556/
• 关键词: 物理療法学; 筋萎縮; 筋障害; 熱刺激; ギプス固定; Heat shock protein; 筋傷害

This study was to determine whether heat stress is preventive for muscle fiber injury induced by reloading after immobilization in rat soleus muscle. Forty-five male 8-week-old Wistar rats were used. Rats were divided randomly into control (C, n=15), immobilized followed by normal recovery (IR, n=15), and immobilized followed by heat stress before reloading (IH, n=15) groups. Bilateral ankles of each rat in the IR and IH groups were fixed in full planter flexion with a plaster cast for 4 weeks. Immediately after 4 weeks of immobilization, reloading was permitted in the IR group. Rats in the IH group were first exposed to heat stress (41 ℃ for 60 min) in an incubator under anesthesia 2 days before reloading. At 0, 3 and 5 days after reloading, both soleus muscles in all groups were excised for histopathological and biochemical analyses. Immediately after remobilization, Hsp70 levels were significantly increased in IH groups compared with IR groups. In 3 days after reloading, the number of necrotic muscle fibers was significantly increased in IR compared with IH groups. The present findings suggest that heat stress prior to reloading induces expression of Hsp70 and leads to protective effect against soleus muscle injury induced by reloading after immobilization.

本研究旨在确定热应激是否可以预防大鼠比目鱼肌固定后重新加载引起的肌纤维损伤，将 45 只 8 周龄雄性 Wistar 大鼠随机分为对照组（C，n=15）。 ）、固定后正常恢复（IR，n=15）和固定后重新加载前热应激（IH，n=15）组 IR 和 IH 组每只大鼠的双侧踝关节。用石膏将大鼠完全屈曲固定4周。固定4周后，IR组大鼠首先在培养箱中接受热应激（41℃，60分钟）。重新加载前2天麻醉下，重新加载后0、3和5天，切除所有组的比目鱼肌进行组织病理学和生化分析。重新活动后，与 IR 组相比，IH 组的 Hsp70 水平显着增加。在重新加载后 3 天，与 IH 组相比，IR 组的坏死肌纤维数量显着增加。目前的研究结果表明，重新加载之前的热应激会诱发这种情况。 Hsp70 的表达并对固定后重新加载引起的比目鱼肌损伤产生保护作用。

项目成果

温熱刺激によるデキサメタゾン誘導性の筋萎縮の進行抑制効果について

热刺激对抑制地塞米松引起的肌肉萎缩进展的作用

• 发表时间: 2009
• 作者: 岩田全広;坂野裕洋;他5名
• 通讯作者: 他5名

Effects of heat stress on muscle fibers damage in reloading after immobilized rat soleus muscle

热应激对大鼠比目鱼肌固定后再加载时肌纤维损伤的影响

• 发表时间: 2007
• 作者: Hara;Yukihiro;Banno Y
• 通讯作者: Banno Y

ギプス固定後の再荷重によるラットヒラメ筋の筋線維損傷に対する温熱負荷の影響

热负荷对大鼠比目鱼肌石膏固定后重新加载引起的肌纤维损伤的影响

• 发表时间: 2009
• 期刊: 理学療法学 36巻2
• 作者: 坂野裕洋;沖田実;他6名
• 通讯作者: 他6名

下腿三頭筋の遅発性筋痛が歩行能力に与える影響

小腿三头肌迟发性肌肉疼痛对步行能力的影响

• 发表时间: 2010
• 作者: 坂野裕洋;松原貴子;岩田全広;浅井友詞;鈴木重行
• 通讯作者: 鈴木重行

運動時の筋に対する温熱作用について

关于运动过程中对肌肉的热效应

• 发表时间: 2010
• 作者: 坂野裕洋;沖田実;他6名;坂野裕洋
• 通讯作者: 坂野裕洋