Development of transplantable self-regenerative liver tissue consisted of hepatocytes and stem cells

开发由肝细胞和干细胞组成的可移植的自我再生肝组织

• 批准号: 21659307
• 负责人: KANEMATSU Takashi
• 金额: $ 2.16万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659307/
• 关键词: 肝細胞; 幹細胞; 自己再生型; 肝細胞移植; 肝移植; 可移植性組織片; 細胞シート; 肝細胞シート

We established the procedures of cultured hepatocyte sheet and fibroblast sheet in this study. Fibroblast sheet was transplantable to the abdominal cavity and showed excellent adaptation in vivo. We have discovered that fibroblast sheet enhanced tissue repair microscopically and induced accumulation of oval cell, a hepatic progenitor cell.We have not got an evidence of hepatic stem cells from fibroblast sheet itself, however, using recipient specific markers, accumulated cells were originated from recipient. Thus, we speculate that fibroblast sheet promote recipient stem cells thorough the improvement of injured recipient environment.In this study, we also have attempted that coculture system of hepatocyte and fibroblast sheet. Technically, it has been achieved, but, the cellular assembly remained mature tissue. Although further study is needed to obtain initial purposes, fibroblast sheet is expecting candidate for the transplantable tissue engineering which promotes stem cells.

本研究建立了肝细胞片和成纤维细胞片的培养方法。成纤维细胞片可移植到腹腔并在体内表现出良好的适应性。我们发现成纤维细胞片在显微镜下增强组织修复并诱导卵圆细胞（一种肝祖细胞）的积累。我们还没有从成纤维细胞片本身获得肝干细胞的证据，但是，使用受体特异性标记物，积累的细胞源自受体。由此，我们推测成纤维细胞片通过改善受损伤的受者环境来促进受体干细胞的生长。在本研究中，我们还尝试了肝细胞与成纤维细胞片的共培养体系。从技术上讲，它已经实现了，但是，细胞组装仍然是成熟的组织。尽管还需要进一步研究才能达到初步目的，但成纤维细胞片有望成为促进干细胞移植的组织工程的候选者。

项目成果

Elective living donor liver transplantation by hybrid hand-assisted laparoscopic surgery and short upper midline laparotomy

• DOI: 10.1016/j.surg.2011.06.021
• 发表时间: 2011-11-01
• 期刊: SURGERY
• 影响因子: 3.8
• 作者: Eguchi, Susumu;Takatsuki, Mitsuhisa;Kanematsu, Takashi
• 通讯作者: Kanematsu, Takashi

Lack of Grafted Liver Rejuvenation in Adult-to-Pediatric Liver Transplantation

• DOI: 10.1007/s10620-010-1445-5
• 发表时间: 2011-05-01
• 期刊: DIGESTIVE DISEASES AND SCIENCES
• 影响因子: 3.1
• 作者: Eguchi, Susumu;Takatsuki, Mitsuhisa;Kanematsu, Takashi
• 通讯作者: Kanematsu, Takashi

Expression of E-cadherin in hepatocellular carcinoma with previously local therapies in the explanted cirrhotic liver for liver transplantation

肝移植肝硬化肝局部治疗后E-钙粘蛋白在肝细胞癌中的表达

• 发表时间: 2011
• 作者: Hidaka M;Takatsuki M;Soyama A;Okudaira S;Muraoka I;Adachi T;Kuroki T;Kanematsu T;Eguchi S
• 通讯作者: Eguchi S

肝再生刺激とIFN signalingからみた肝移植後IFN開始至適時期の検討

从刺激肝再生和IFN信号传导角度探讨肝移植后启动IFN的最佳时机

• 发表时间: 2009
• 作者: 濱崎幸司;江口晋;蒲原行雄;高槻光寿;山之内孝彰;宮崎健介;猪熊孝実;朝長哲生;黒木保;田島義証;兼松隆之
• 通讯作者: 兼松隆之

高齢肝細胞癌患者に対する肝切除の検討

老年肝细胞癌患者行肝切除术的思考

• 发表时间: 2009
• 作者: 山之内孝彰;江口晋;高槻光寿;蒲原行雄;宮崎健介;猪熊孝実;朝長哲生;黒木保;田島義証;兼松隆之
• 通讯作者: 兼松隆之