Console and probe heads 600 MHz NMR spectrometer

控制台和探头 600 MHz NMR 波谱仪

• 批准号: 539080547
• 金额: --
• 依托单位: Universität Konstanz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/539080547?language=en
• 关键词: Console; probe; heads; 600; MHz

The major equipment applied for aims to enable the execution of research projects at the NMR core facility that possess highest relevance. With this investment the application spectrum modern high-resolution NMR spectroscopy offers will be significantly expanded as the two heteronuclei fluorine (19F) and phosphor (31P) will be then available at a 600 MHz NMR machine. Currently, highly sensitive 19F NMR experiments can be exclusively conducted on an 800 MHz NMR machine (equipped with CP-QCI probe). It has been shown that using a field strength of 600 MHz depicts an excellent experimental setup to acquire 19F NMR data due to the intrinsic characteristics 19F possesses. Besides, experimental data on 31P nuclei cannot be acquired on the 600 MHz NMR machine at the NMR core facility at the moment. Extensive studies on nucleotides that are based on NMR spectroscopic approaches rely on the experimental accessibility of 31P nuclei. Consequently, the major equipment applied for crucially expands the implementation range enabling to tackle research objectives pursued in DNA/RNA research. Research groups affiliated with the Department of Chemistry or Biology at the University of Konstanz are strongly involved in collaborative efforts promoting interdisciplinarity. Here, an appropriate up to date equipped NMR infrastructure strongly supports research projects that rely on experimental insights at the molecular and even atomic scale. Exemplarily, fluorine-based studies aiming to determine thermodynamic stability and function of proteins in a cellular context, projects on posttranslational modifications of proteins and complementary studies combining computer simulations with NMR spectroscopic experimental data can be listed. Another motivation for the investment in the major equipment applied for concerns the availability of the instruments provided by the NMR core facility. Currently, two cryo platforms are operated (600 MHz equipped with CP-TCI cryo probe (installation in 2008) as 800 MHz equipped with CP-QCI cryo probe (installation in 2009)). Using state-of-the-art room temperature probe heads increases the robustness, dependability and, significantly, the temperature range that can be used for experimental efforts at the 600 MHz NMR spectrometer. The existing superconducting magnet possessing magnetic field strength of 14.1 T (installation in 2008) will be kept and further used. The major equipment applied for will compensate the transition from a cryo platform to a room temperature setup due to technical advancement and methodological progress of probe heads and console technology noted during the past 15 years. In parallel, the experimental availability of NMR active nuclei will be significantly expanded (currently: 1H, 2H, 13C, 15N; then: 1H, 2H, 13C, 15N, 19F und 31P).

主要设备旨在使具有最高相关性的NMR核心设施的研究项目执行。通过这项投资，随着两种异核氟（19F）（19F）和Phosphor（31p）将在600 MHz NMR机器上提供，应用频谱现代高分辨率NMR光谱率提供的提供将大大扩展。当前，高度敏感的19F NMR实验可以在800 MHz NMR机器（配备CP-QCI探针）上进行。已经表明，使用600 MHz的场强描绘了出色的实验设置，以获取19F NMR数据，因为固有特征19F拥有。此外，目前在NMR核心设施的600 MHz NMR机器上无法获取31p核的实验数据。基于NMR光谱方法的核苷酸的广泛研究取决于31p核的实验可及性。因此，适用于至关重要的设备扩大了实施范围，从而解决了DNA/RNA研究中追求的研究目标。康斯坦茨大学化学或生物学系的研究小组强烈参与促进跨学科性的合作努力。在这里，适当的最新配备的NMR基础设施强烈支持依赖于分子甚至原子量表实验见解的研究项目。示例性的基于氟的研究旨在确定细胞环境中蛋白质的热力学稳定性和功能，可以列出蛋白质翻译后修饰的项目以及将计算机模拟与NMR光谱实验数据相结合的互补研究。对主要设备进行投资的另一个动机是涉及NMR核心设施提供的工具的可用性。目前，运行两个冷冻平台（配备CP-TCI冷冻探针的600 MHz（2008年安装）为800 MHz，配备了CP-QCI冷冻探针（2009年安装）。使用最先进的室温探针头可以提高可靠性，可显着的温度范围，可用于在600 MHz NMR光谱仪上用于实验性工作。现有的具有14.1 T的磁场强度（2008年安装）的超导磁铁将保留并进一步使用。应用的主要设备将弥补从冷冻平台到室温设置的过渡，这是由于过去15年中提到的探针头和控制台技术的技术进步和方法论进步。同时，NMR活性核的实验可用性将显着扩展（目前：1H，2H，13C，15N；然后：1H，2H，13C，13C，15N，19F UND 31P）。