Epigenetic regulation of VEGF-C/-D in cervical lymph node metastasis.

VEGF-C/-D在颈部淋巴结转移中的表观遗传调控。

• 批准号: 21791967
• 负责人: SHINOHARA Fumiaki
• 金额: $ 2.83万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791967/
• 关键词: 口腔外科学一般(含病態検査学); 癌; エピジェネティクス; 血管新生; リンパ節転移

In this study, we used the novel DNA methyltransferase inhibitor zebularine (Zeb) to investigate epigenetic influences on the secretion of VEGF-A in the OSCC cell line HSC-3. Our study revealed that neither the PI3K/Akt nor the p53 signaling pathway is required for Zeb-induced hypoxia-inducible factor-1α (HIF-1α) degradation.In addition, we investigated the effects of EGFR antagonist Cetuximab on apoptosis induced by anti-cancer drug(5-FU or CDDP )in HSC-3 cells. In this study, we found that Cetuximab significantly enhanced the apoptosis of HSC-3 or HSC-4 cells treated by 5-FU, but not CDDP. These results suggested the mechanism depends on the PI3K/Akt signaling pathway.

在这项研究中，我们的USDA甲基转移酶抑制剂（ZEB）在OSCC细胞系HSC-3中研究表观遗传学，我们的研究表明，PI3K/AKT和P53信号传导均不需要ZEB诱导的低氧诱导途径因子1α（HIF-1α）降解。在此研究中，HSC-3细胞中抗癌药物（5-FU或CDDP）诱导的凋亡中的EGFR拮抗剂Cetuximab。 HSC-3或通过5-FU处理的细胞，但不取决于PI3K/AKT信号通路。

项目成果

Enhancement of cisplatin cytotoxicity by SAHA involves ER stress-mediated apoptosis in OSCC.

SAHA 增强顺铂细胞毒性涉及 OSCC 中内质网应激介导的细胞凋亡。

• 发表时间: 2010
• 作者: Suzuki M;Shinohara F;Endo M;Sugazaki M;Echigo S;Rikiishi H.;Maiko Suzuki;遠藤学
• 通讯作者: 遠藤学

Enhancement of cisplatin cytotoxicity by SAHA involves endoplasmic reticulum stress-mediated apoptosis in oral squamous cell carcinoma cells

• DOI: 10.1007/s00280-009-0969-x
• 发表时间: 2009-03
• 期刊: Cancer Chemotherapy and Pharmacology
• 影响因子: 3
• 作者: Maiko Suzuki;Manabu Endo;F. Shinohara;S. Echigo;H. Rikiishi

Suppressive effects of Zebularine on 5-FU sensitivity in cancer cells

Zebularine 对癌细胞 5-FU 敏感性的抑制作用

• 发表时间: 2009
• 作者: Maiko Suzuki;Manabu Endo;Fumiaki Shinohara
• 通讯作者: Fumiaki Shinohara

Up-regulation of 5-FU sensitivity by Cetuximab in oral squamous cell carcinoma

西妥昔单抗上调口腔鳞状细胞癌中的 5-FU 敏感性

• 发表时间: 2009
• 作者: Manabu Endo;Maiko Suzuki;Fumiaki Shinohara
• 通讯作者: Fumiaki Shinohara