Role of heat-shock proteins in the ocular immune privilege

热休克蛋白在眼部免疫豁免中的作用

• 批准号: 21791671
• 负责人: TAKASE Hiroshi
• 金额: $ 2.58万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791671/
• 关键词: 免疫学; 眼科; 炎症; 熱ショック蛋白; 前房水; サイトカイン; 眼免疫; 眼内液; ぶどう膜炎

The present study was aimed at investigating the role of heat-shock proteins (HSPs) in the immune regulation in the eye. We hypothesized that the intraocular fluids contain secreted form of HSPs, and that the HSPs act as antigens targeted by ocular infiltrating cells, or as immune suppressive agents. We first tested if aqueous humor from patients with cataract contain HSPs. We detected HSP27 in the aqueous humor, whereas HSP10, HSP60, or HSP70 were not detected. We then co-cultured recombinant HSP27 with T cell clones (TCCs) and tested if HSP27 has effects on the proliferation or cytokine production of TCCs. As a result, HSP27 up-regulated interferon-gamma production by TCCs.

本研究旨在研究热休克蛋白（HSP）在眼睛免疫调节中的作用。我们假设眼内液含有分泌形式的热休克蛋白，并且热休克蛋白充当眼部浸润细胞靶向的抗原，或作为免疫抑制剂。我们首先测试了白内障患者的房水中是否含有热休克蛋白。我们在房水中检测到 HSP27，而未检测到 HSP10、HSP60 或 HSP70。然后，我们将重组 HSP27 与 T 细胞克隆 (TCC) 共培养，并测试 HSP27 是否对 TCC 的增殖或细胞因子产生有影响。结果，HSP27 上调了 TCC 的干扰素 γ 产生。