Investigation on the significance of vascular endothelial growth factor-A in the pathology of osteoarthritis.

血管内皮生长因子-A在骨关节炎病理学中的意义探讨

• 批准号: 21791426
• 负责人: KONAGI Tanaka
• 金额: $ 2.75万
• 依托单位: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791426/
• 关键词: 変形性関節症; 血管内皮細胞増殖因子

The result of gene expression analysis in cartilage and clinical experience suggested possible involvement of VEGF-A in the pathology of osteoarthritis (OA). This study was conducted to examine the possibility of the involvement of VEGF-A in the synovial change observed with the disease. I first performed compression experiments with cartilage tissues. For this, cartilage tissues were obtained from OA and control knee joints, and compressive force was given to the tissues repeatedly to simulate weight bearing situation in the actual daily activities. The amount of pressure was adjusted to the level that cartilage tissues undergo in level walking (1 MPa). The result of this experiment revealed that cartilage tissues from OA knees release considerable amounts of VEGF-A upon physiological levels of compression. The amount of VEGF-A released from the tissue was significantly related to the severity of degeneration. Since this result suggested possible involvement of VEGF-A in the synovial changes occurs within OA joints, gene expression analysis was conducted with OA synovium. For this, synovial tissues were obtained from end-stage OA knees, and the levels of gene expression related to VEGF-A were determined by qPCR. However, the result of this analysis did not support our hypothesis that VEGF-A released from cartilage tissues induces synovial change. Thus, from the result of this study, it was concluded that VEGF-A might be released from degenerated cartilage, but it does not play a significant role in the pathology of OA, at least in terms of synovial pathology.

在软骨和临床经验中基因表达分析的结果表明，VEGF-A可能参与骨关节炎（OA）的病理。进行了这项研究是为了检查VEGF-A参与该疾病的滑膜变化的可能性。我首先对软骨组织进行了压缩实验。为此，从OA和对照膝关节获得了软骨组织，并反复给组织以模拟组织中的压缩力，以模拟实际日常活动中的负重轴承情况。将压力量调节到软骨组织在水平行走（1 MPa）中经历的水平。该实验的结果表明，OA膝盖的软骨组织在压缩的生理水平下释放了相当多的VEGF-A。从组织释放的VEGF-A量与变性的严重程度显着相关。由于该结果表明VEGF-A可能参与滑膜变化，因此在OA关节内发生，因此使用OA滑膜进行了基因表达分析。为此，从末期OA膝盖获得了滑膜组织，并通过qPCR确定与VEGF-A相关的基因表达水平。但是，该分析的结果不支持我们的假设，即从软骨组织释放的VEGF-A会引起滑膜变化。因此，从这项研究的结果中得出结论，VEGF-A可能会从变性软骨中释放出来，但至少在滑膜病理学方面，它在OA的病理中并不重要。

项目成果

Progression of symptomatic medial knee osteoarthritis. Relationship between radiographic changes and symptoms.over a 3-year period.

有症状的内侧膝骨关节炎的进展。

• 发表时间: 2010
• 作者: Naoshi Fukui;Shoji Yamane;Nobuho Tanaka;Riako Masuda;Konagi Tanaka;Sei-ichi Azuma;Kimio Masuda;Takuo Juji,Toshihito Mori;Yozo Katsuragawa.
• 通讯作者: Yozo Katsuragawa.

内側型変形性膝関節症の進行過程 -保存療法例におけるレントゲン、愁訴、臨床所見の関連-

膝关节内侧骨关节炎的进展过程 - 保守治疗病例中 X 光、主诉和临床表现之间的关系 -

• 发表时间: 2010
• 作者: 福井尚志;山根昌治;田中信帆;増田理亜子;田中こなぎ;十字琢夫;森俊仁;桂川陽三
• 通讯作者: 桂川陽三

内側型変形性膝関節症の進行過程 -X-pと愁訴、臨床所見の関連の検討-

膝关节内侧骨关节炎的进展过程 - X-p、主诉和临床表现之间关系的检查 -

• 发表时间: 2010
• 作者: 福井尚志;山根昌治;田中信帆;増田理亜子;田中こなぎ;東成一;十字琢夫;桂川陽三;森俊仁
• 通讯作者: 森俊仁

内側型膝OAの進行過程 -保存的治療例におけるレントゲン、愁訴、臨床所見の経時的変化-

膝关节内侧 OA 的进展过程 - 保守治疗病例中 X 光、主诉和临床表现随时间的变化 -

• 发表时间: 2009
• 作者: 福井尚志;山根昌治;田中信帆;増田理亜子;増田公男;田中こなぎ;東成一;十字琢夫;森俊仁;桂川陽三
• 通讯作者: 桂川陽三