The analysis of neuronal mechanism of the nucleus in the midbrain related to calling behavior with sexual difference in birds

鸟类中脑核与性别差异叫声相关的神经机制分析

基本信息

批准号：
13680879
负责人：
AOKI Kiyoshi
金额：
$ 2.11万
依托单位：
Sophia University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680879/
关键词：
Bengalese finches dorsomedial nucleus(DM)sexual difference distance call(DC)robust nucleus of the archistriatum (RA)patch clamp recording sensory motor learning stage after crystallization stage distance call(DC)音声パターンスライス標本

项目摘要

The purpose of this study is to clarify the neural function and anatomical structure at the cellular level in network of the dorsomedial nucleus(DM) in the vocal control system and in the producing of sexually different acoustic structures of distance calls(DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at the cellular level rec … More ording of the impulses of neurons in the robust nucleus of the archistriatum (RA) by cell patch clamp.In this study, whole cell patch clamp recordings were made from neurons of the slice preparation in the RA, which is a motor control nucleus of male-specific vocalization in male birds of different developmental stage. We focused on the firing patterns in the neural activity of RA neurons, and also focused on the difference in neural activity between the "sensory motor learning" stage and the "after crystallization" stage. The neurons in the RA were categorized into three types according to their electrophysiological properties under voltage clamp recording. The three types are "non-spike", "single-spike" and "multi-spike" type neurons. Distribution ratios of the three types changed during developments. We compared the distribution of the three types of these neurons in the two different learning stages. The distribution ratios of multi-spike type neuron was significantly higher than that of the single-spike type neuron in the sensory motor learning stage, and the relationship between the distribution ratios was reversed in the after crystallization stage. No significant difference between the distribution ratios of the non-spike type neuron in the two learning stages was observed Less

本研究的目的是阐明声音控制系统中背内侧核（DM）网络的神经功能和细胞水平的解剖结构以及本能行为的远距离呼叫（DC）产生性别不同的声音结构的神经功能和解剖结构。在性成熟的孟加拉雀（Lonchura striata var. Domestica）中，未成熟的雄性和雌性具有相似的远距离叫声，而在性成熟的雄性中，远距离叫声的声学结构表现出性别差异，这种行为二分法是由于神经支配所致。当雄性的无毛核（RA）发育成性成熟时，从 RA 到 DM 的神经输入上述结果是对性成熟雄性双侧 RA 电解损伤前后的距离叫声结构进行分析的结果。在双侧 RA 损伤中，这些雄性产生了与雌性类似的声音，我们在细胞水平上通过细胞膜片钳研究了古纹状体 (RA) 强核中神经元脉冲的排序。 ,全细胞膜片钳记录是由 RA 中的切片神经元进行的，RA 是不同发育阶段雄性鸟类中雄性特异性发声的运动控制核，我们重点关注 RA 神经元神经活动的放电模式。还重点研究了“感觉运动学习”阶段和“结晶后”阶段神经活动的差异，根据电压钳记录下的电生理特性将RA中的神经元分为三种类型。 “非尖峰”，我们比较了这三种类型神经元在两个不同学习阶段的分布情况。在感觉运动学习阶段，单尖峰型神经元的分配比例之间的关系发生了逆转，而在结晶后阶段，非尖峰型神经元的分配比例没有显着差异。观察到两个学习阶段 Less