The Analysis of Neuronal Mechanism of Vocal Nucleus related to Testosterone in Passerine.

雀形目声带核与睾酮相关的神经机制分析。

基本信息

批准号：
11680793
负责人：
AOKI Kiyoshi
金额：
$ 2.37万
依托单位：
Sophia University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The purpose of this study is to clarify the physiological function and anatomical structure of the dorsomedial nucleus (DM) in the vocal control system and in the producing of sexually different acoustic strtuctures of distance calls (DC) of instinct behavior in sexually mature Bengalese finches (Lonchura striata var. domestica) Immature males and females have acoustically similar distance calls and in sexually mature male under the effect of testosterone, sex hormone, the acoustic structures of distance calls show sexual differences. This behavioral dichotomy is due to innervation of the neural input from RA to DM when robust nucleus of the arichistriatum (RA) in the male developed sexually mature. The above findings are results of analyzing acoustic structures of distance calls before and after electrolytic lesions of the bilateral RA in sexually mature males. After bilateral RA lesioning, these males have produced distance calls acoustically similar to those of female. We studied at … More the cellular level recording of the impulses of neurons in DM by microelectrode. The response patterns of spike discharges from neurons in DM of males and females did not show sexual difference. Mean impulse number of spike discharges increased just before vocalization of distance call.We investigated microstructure of the nervous system of DM in mature male by anatomical method. The synapse of anterograde labeling with neural tracers was observed to be excitable synapse. The characteristic structure of DM in male has axonal input of nerve connection from RA to the dorsoventral side in DM and this axonal input connected the neuronal circuit related to producing of distance calls.We investigated response patterns from the results of multi-unit recording of spike discharges from neuron in DM of mature males and females when we stimulated left and right acoustic organs by the method of playback of DC.The duration of the firing spike discharges producing distance call in male is longer than that in female. Also the number of impulse frequencies in male is higher than that in female.These results suggest that axonal input of neuronal connection from the RA to DM of sexually mature male modulates the acoustic structure of its DC.We clarified by neuroethological studies that the neural input from the RA to DM was due to action of testosterone, sex hormone. Less

本研究的目的是阐明背内侧核（DM）在发声控制系统中的生理功能和解剖结构，以及在性成熟孟加拉雀本能行为的远距离叫声（DC）中产生性别不同的声学结构。 Lonchura striata var. Domestica）未成熟的雄性和雌性具有相似的远距离叫声，而性成熟的雄性在睾酮、性激素的作用下，远距离叫声的声学结构表现出性别差异。当雄性的无毛核（RA）发育到性成熟时，从 RA 到 DM 的神经输入的神经支配上述结果是分析性交时双侧 RA 电解损伤前后距离叫声的声学结构的结果。在双侧 RA 损伤后，这些雄性发出与雌性类似的声音，我们在细胞水平上通过微电极记录了 DM 中神经元的脉冲放电的反应模式。男性和女性的DM没有表现出性别差异。在发出远距离叫声之前，尖峰放电的平均脉冲数增加。我们通过解剖学方法研究了成熟男性DM的神经系统的微观结构，用神经示踪剂顺行标记了突触。观察到男性 DM 的特征结构是从 RA 到 DM 背腹侧的神经连接的轴突输入，该轴突输入连接与产生相关的神经元回路。我们通过对成熟雄性和雌性 DM 神经元尖峰放电的多单元记录结果，研究了当我们通过 DC 回放的方法刺激左右声器官时的反应模式。男性产生距离呼叫的放电比女性长，并且男性的脉冲频率数量比女性高。这些结果表明，性成熟男性从 RA 到 DM 的神经连接的轴突输入调节声学结构。其 DC.We神经行为学研究表明，从 RA 到 DM 的神经输入是由于睾酮、性激素的作用较少。