Identification of MURC, muscle-restricted coiled-coil protein, and molecular characterization of MURC-induced cardiac dysfunction and arrhythmia

MURC、肌肉限制性卷曲螺旋蛋白的鉴定以及 MURC 诱导的心功能障碍和心律失常的分子特征

• 批准号: 21790740
• 负责人: TAGAWA Masashi
• 金额: $ 2.75万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790740/
• 关键词: 心筋細胞; 心不全; 不整脈; MURC

We identified a muscle-restricted coiled-coil protein, MURC, and found that MURC was localized to caveolae at the plasma membrane of cardiomyocytes and was associated with caveolin-3, PTRF/cavin-1, SDPR/cavin-2, and SRBC/cavin-3. We also found that overexpression of MURC in cardiomyocytes induced distension of caveolae and transgenic mice expressing MURC in the heart exhibited deformation of caveolae in cardiomyocytes. Since transgenic mice expressing MURC in the heart shows cardiac dysfunction and arrhythmia, we are investigating the molecular mechanisms responsible for MURC-induced cardiac dysfunction and arrhythmia.

我们鉴定了一种肌肉限制性卷曲螺旋蛋白 MURC，并发现 MURC 定位于心肌细胞质膜的小窝，并与 Caveolin-3、PTRF/cavin-1、SDPR/cavin-2 和 SRBC/卡文-3。我们还发现，心肌细胞中MURC的过度表达引起小窝扩张，并且在心脏中表达MURC的转基因小鼠表现出心肌细胞小窝变形。由于心脏表达 MURC 的转基因小鼠表现出心脏功能障碍和心律失常，因此我们正在研究 MURC 诱导的心脏功能障碍和心律失常的分子机制。

项目成果

A novel but evolutionarily conserved muscle-restricted coiled-coil protein, MURC, regulates myogenic differentiation in C2C12 cells.

MURC 是一种新型但进化上保守的肌肉限制性卷曲螺旋蛋白，可调节 C2C12 细胞的肌原性分化。

• 发表时间: 2008
• 作者: Tagawa M;Ueyama T;Ogata T;Nakajima N;Isodono K;Asada S;Takehara N;Takahashi T;Matsubara Hiroaki;Oh H.
• 通讯作者: Oh H.

A novel muscle-restricted coiled-coil protein, MURC, induces cardiac dysfunction accompanied with an altered gene expression profile.

一种新型肌肉限制性卷曲螺旋蛋白 MURC 可诱导心脏功能障碍，并伴有基因表达谱的改变。

• 发表时间: 2008
• 作者: Ogata T;Ueyama T;Isodono K;Tagawa M;Takehara N;Kawashima T;Shioi T;Takahashi T;Matsubara Hiroaki;Oh H.
• 通讯作者: Oh H.

MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis.

• DOI: 10.1152/ajpcell.00188.2008
• 发表时间: 2008-08
• 期刊: American journal of physiology. Cell physiology
• 作者: M. Tagawa;T. Ueyama;T. Ogata;N. Takehara;Norio Nakajima;K. Isodono;S. Asada;Tomosaburo Takahashi;H. Matsubara;H. Oh