Regulation of translation machinery and mRNA localization by PAR-aPKC complex

PAR-aPKC 复合物对翻译机制和 mRNA 定位的调节

• 批准号: 21770190
• 负责人: HAYASHI Kenji
• 金额: $ 2.91万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770190/
• 关键词: PAR1b; MARK2; Microtubule; Dendritic spine; +TIPs; PAR1b/MARK2; PAR-aPKC; mRNA; PAR-1b

We revealed that PAR1b participates in the maintenance of mature dendritic spine morphology in mouse hippocampal neurons. PAR1b localized in the dendrites of mature neurons, and PAR1b knockdown cells exhibited decreased mushroom-like dendritic spines, as well as increased filopodia-like dendritic protrusions. We revealed that microtubule growth distance in dendrite region is decreased following PAR1b knockdown. In addition, reduced accumulation of GFP-p140Cap, an actin reorganizing protein, in dendritic protrusions was confirmed in PAR1b knockdown neurons. In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines.

我们透露，PAR1B参与小鼠海马神经元中成熟的树突状脊柱形态的维持。 PAR1B位于成熟神经元的树突中，而PAR1B敲低细胞表现出蘑菇状的树突状刺的降低，并增加了类似丝状的树突状突起。我们透露，在PAR1B敲低后，树突区域的微管生长距离降低。此外，在PAR1B敲低神经元中证实了肌动蛋白重组蛋白的GFP-P140CAP的积累，肌动蛋白的重组蛋白。总之，目前的结果表明，通过调节微管生长和p140CAP在树突状棘中的积累，PAR1B在维持成熟的树突状形态中具有新的功能。

项目成果

Maintenance of dendritic spine morphology by PAR1b through regulation of microtubule dynamics

PAR1b 通过调节微管动力学维持树突棘形态

• 发表时间: 2011
• 作者: 林健二