Elucidation of neuronal regenerative mechanism after ischemia and its modulation therapy

缺血后神经元再生机制的阐明及其调节治疗

• 批准号: 21390413
• 负责人: KAWAHARA Nobutaka
• 金额: $ 11.32万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390413/
• 关键词: 脳虚血; 神経細胞死; 神経再生; 線条体; 軸索進展; 電気生理; 成長因子

Here we explored whether neuronal regeneration can be augmented by administration of exogenous growth factors by using rat global ischemia model, which induced selective neuronal death in the striatum. As a result, neuronal density in the striatum decreased to 1% by 2 days after ischemia, that was finally increased to 12-15% by the growth factor treatment. When dividing neural progenitors were labeled by BrdU, the progeny of these cells were distributed in the striatum, differentiated into region specific projection and inter-neurons. In addition, these cells maturated electrophysiologically after 20 weeks of ischemia. Motor performance also improved significantly. Collective, these results suggested that the modulation of endogenous neuronal regeneration can be potentiated by the treatment, and finally leads to functional recovery.

在这里，我们探讨了通过使用大鼠全球缺血模型的给药外源生长因子可以增加神经元再生，从而诱导纹状体中的选择性神经元死亡。结果，缺血后2天，纹状体的神经元密度降至1％，最终通过生长因子处理增加到12-15％。当分裂神经祖细胞被BRDU标记时，这些细胞的后代分布在纹状体中，分化为特定区域的投影和神经元。另外，这些细胞在缺血20周后在电生理上成熟。运动性能也有了显着改善。这些结果集体表明，该处理可以增强内源性神经元再生的调节，最后导致功能恢复。

项目成果

Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by grwoth factors

生长因子诱导缺血后纹状体神经发生和区域特异性功能成熟神经元的产生

• 发表时间: 2010
• 期刊: J Neurosurg
• 影响因子: 4.1
• 作者: Yoshikawa G;Momiyama T;Oya S;Takai K;Tanaka J;Higashiyama S;Saito N;Kirino T;Kawahara N
• 通讯作者: Kawahara N

Intrinsic neurogenesis in cerebral ischmemia

脑缺血的内在神经发生

• 发表时间: 2011
• 作者: Takewa Y;Nakayama Y;Yamanami M;Kishimoto Y;Arakawa M;Ohnuma K;Sumikura H;Kanda K;Matsui Y;Taenaka Y;Tatsumi E;Kawahara N
• 通讯作者: Kawahara N

虚血性脳血管障害に対する神経再生療法

缺血性脑血管病的神经再生治疗

• 发表时间: 2010
• 作者: Oishi M;他;川合謙介;川原信隆
• 通讯作者: 川原信隆

Induction of striatal neurogenesis and generation of region-specific functional mature neurons after ischemia by grwoth factors.

生长因子在缺血后诱导纹状体神经发生和区域特异性功能成熟神经元的生成。

• 发表时间: 2010
• 期刊: J Neurosurg
• 影响因子: 4.1
• 作者: Yoshikawa G;Momiyama T;Oya S;Takai K;Tanaka J;Higashiyama S;Saito N;Kirino T;Kawahara N
• 通讯作者: Kawahara N

脳虚血と再生に関する研究

脑缺血与再生研究

• 发表时间: 2011
• 作者: Yoshida D;Koketshu K;Nomura R;Teramoto A;川原信隆
• 通讯作者: 川原信隆