Analysis of pathogenesis and pathophysiology of chronic kidney disease glomerulus by microproteomics

微蛋白质组学分析慢性肾病肾小球的发病机制和病理生理学

• 批准号: 21390262
• 负责人: YAMAMOTO Tadashi
• 金额: $ 10.9万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390262/
• 关键词: 慢性腎臓病; 糸球体; プロテオーム; 質量分析計; 抗体; データベース

Primary pathological lesion of chronic kidney disease(CKD) is the glomerulus. The CKD is paid a serious attention in medicine as a part of CKD patients will lose their kidney function in the future and need dialysis treatment or kidney transplantation. This disease is classified into several diseases by pathological examination, however, the pathogenesis and pathophysiology are almost unknown, which make it difficult to develop curative treatments for CKD.The current study was aimed to analyze glomerular tissue samples obtained by kidney biopsy from CKD patients to understand the pathogenesis and pathophysiology by bioinformatics. At first, comprehensive proteome of normal human glomerulus was examined by proteomics using mass spectrometers(MS) and antibodies to know normal human glomerulus proteome. The glomeruli were isolated by the sieving method from kidneys removed from patients with renal carcinomas. More than 3000-gene-derived proteins were identified in the glomerulus and the results are opened for public through a website as a Human Glomerulus Proteome Database(http ://www. hkupp. org).Secondary, MS proteomics was applied to frozen or formalin-fixed paraffin-embedded(FFPE) kidney biopsy samples. Fifty glomerular sections were practically collectable by a laser micro dissection method from each kidney biopsy specimen and the amount of proteins or peptides

慢性肾脏疾病（CKD）的主要病理病变是肾小球。由于CKD患者的一部分将在将来失去肾脏功能，并且需要透析治疗或肾脏移植。通过病理检查将这种疾病分为几种疾病，但是，发病机理和病理生理学几乎未知，这使得很难为CKD开发治愈性治疗。目前的研究旨在分析CKD患者肾脏活检获得的肾小球组织样本了解生物信息学的发病机理和病理生理学。首先，使用质谱仪（MS）和抗体了解正常的人肾小球蛋白质组，通过蛋白质组学检查了正常人肾小球的全面蛋白质组。通过从肾癌患者中去除的肾脏的筛分方法分离肾小球。在肾小球中鉴定了超过3000种基因的蛋白质，并以人肾小球蛋白质组数据库（http：//www。hkupp.org）为公众开放结果。福尔马林固定石蜡包裹（FFPE）肾脏活检样品。实际上，通过从每个肾脏活检标本和蛋白质或肽的量，可以通过激光微剖析方法收集五十个肾小球切片

项目成果

On-site Proteomics Study on Laser Microdissected Rat Renal Cortex and Human Glomerulus Tissues

激光显微切割大鼠肾皮质和人肾小球组织的现场蛋白质组学研究

• 发表时间: 2010
• 作者: Xu B;Shimada S;Zhang Y;Magdaldin S;ujinaka H;Yoshida Y;Yaoita E;Yamamoto T
• 通讯作者: Yamamoto T

Final standard protocol for non proteinuric urine proteomics 4^<th> HKUP Workshop in HUPO 2009 Annual Congress"Proteomics for discovery of urine biomarkers"

非蛋白尿尿液蛋白质组学最终标准方案 HUPO 2009 年会上第四届 HKUP 研讨会“蛋白质组学发现尿液生物标志物”

• 发表时间: 2009
• 作者: Miyazaki K;Ohta Y;Morimoto N;Kurata T;Takehisa Y;Ikeda Y;Matsuura T;Abe K;Shoichi Maruyama;Yoshida Y
• 通讯作者: Yoshida Y

Proteomics for Discovery of Urine Biomarkers

用于发现尿液生物标志物的蛋白质组学

• 发表时间: 2009
• 作者: Rie Matsumori;Takashi Matsuzaka;Yukio Nagasaki;Hitoshi Shimano;et al.;神田隆;Yamanaka K;鮎澤信宏;金井好克;森啓;Tadashi Yamamoto
• 通讯作者: Tadashi Yamamoto

尿中インスリン様成長因子結合蛋白(IGFBP)の意義

尿胰岛素样生长因子结合蛋白（IGFBP）的意义

• 发表时间: 2010
• 作者: 藤中秀彦;矢尾板永信;吉田豊;富沢修一;山本格
• 通讯作者: 山本格

Proteomics for Understanding of Kidney Functions and Diseases

用于了解肾脏功能和疾病的蛋白质组学

• 发表时间: 2010
• 作者: Morizane R;Monkawa T;Konishi K;Hashiguchi A;Ueda M;Ando Y;TokuyamaH;Hayashi K;Hayashi M;Itoh H;Yamamoto Tadashi
• 通讯作者: Yamamoto Tadashi