Application of iPS cells to the investigation of the pathogenesis of human retroviral infectious diseases and to the establishment of their prevention and therapy

iPS细胞在人类逆转录病毒感染性疾病发病机制研究及其预防和治疗中的应用

• 批准号: 21390144
• 负责人: SAKAI Koji
• 金额: $ 12.15万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390144/
• 关键词: エイズ; HIV; 移植・再生医療; ウイルス; 感染症; AIDS

We carried out this study in order to obtain basic information on the prevention of virus infection by novel therapies and on the development of therapeutic drugs against human retroviral infectious diseases utilizing human and macaque iPS cells. We introduced the HIV-1 gp160 membrane glycoprotein gene into human iPS cells, and obtained high expression of the gene. Remarkable cellular immunity was induced in HIV-1 gp160-iPS cells-inoculated mice. Thus we showed the feasibility of human iPS cells as a vector with superior performance for novel AIDS vaccines. We introduced into human iPS cells modified cellular factors engineered to inhibit the propagation of HIV, and succeeded to establish cell lines constitutively expressing either of the genes. We established iPS cells from rhesus monkey dermal fibroblasts, and succeeded to differentiate them to stem cells of blood lineage.

我们进行了这项研究，以获取有关通过人类和猕猴IPS细胞对人类逆转录病毒感染疾病的预防病毒感染的基本信息。我们将HIV-1 GP160膜糖蛋白基因引入了人IPS细胞，并获得了该基因的高表达。 HIV-1 GP160-IPS细胞接种的小鼠诱导了明显的细胞免疫。因此，我们显示了人IPS细胞作为新型艾滋病疫苗具有出色性能的载体的可行性。我们引入了人IPS细胞，修饰了设计用于抑制HIV传播的细胞因子，并成功地建立了组成性表达任何一种基因的细胞系。我们从恒河猴皮肤成纤维细胞中建立了IPS细胞，并成功地将它们区分化为血统干细胞。

项目成果

Molecular epidemiology of attachment glycoprotein(G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/2010

2009/2010年日本急性呼吸道感染患儿呼吸道合胞病毒附着糖蛋白(G)基因分子流行病学分析

• DOI: 10.1099/jmm.0.041137-0
• 发表时间: 2012
• 期刊: Journal of Medical Microbiology
• 影响因子: 3
• 作者: Ayako Yoshida1;Naoko Kiyota;Miho Kobayashi;Koichi Nishimura;Rika Tsutsui;Hiroyuki Tsukagoshi;Eiko Hirano;Norio Yamamoto;Akihide Ryo;Mika Saitoh;Seiya Harada;Osamu Inoue;Kunihisa Kozawa;Ryota Tanaka;Masahiro Noda;Nobuhiko Okabe;Masato Tashi
• 通讯作者: Masato Tashi

Molecular epidemiological study of human rhinovirus species A, B and C from patients with acute respiratory illnesses in Japan

• DOI: 10.1099/jmm.0.035006-0
• 发表时间: 2012-03-01
• 期刊: JOURNAL OF MEDICAL MICROBIOLOGY
• 影响因子: 3
• 作者: Arakawa, Mika;Okamoto-Nakagawa, Reiko;Kimura, Hirokazu
• 通讯作者: Kimura, Hirokazu

Vaccination with human induced pluripotent stem cells creates an antigen-specific immune response against HIV-1 gp160

人类诱导多能干细胞疫苗接种可产生针对 HIV-1 gp160 的抗原特异性免疫反应

• DOI: 10.3389/fmicb.2011.00027
• 发表时间: 2011
• 期刊: Frontiers in Microbiology
• 影响因子: 5.2
• 作者: Shinji Yoshizaki;Mayuko Nishi;Asami Kondo;Yoshitsugu Kojima;Naoki Yamamoto;Akihide Ryo
• 通讯作者: Akihide Ryo

Effects of DNA Binding of Zinc Finger and Linkers for Domain Fusion on Catalytic Activity of Sequence-Specific Chimeric Recombinases Determined by a Facile Fluorescent System

锌指和连接体的 DNA 结合对结构域融合对通过简易荧光系统测定的序列特异性嵌合重组酶催化活性的影响

• DOI: 10.1021/bi201878x
• 发表时间: 2012
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Nomura;W.;Masuda;A.;Ohba;K.;Urabe;A.;Ito;N.;Ryo;A.;Yamamoto;N.;Tamamura;H.
• 通讯作者: H.

Identification of phosphorylated proteins involved in the oncogenesis of prostate cancer via Pin1-proteomic analysis

• DOI: 10.1002/pros.21466
• 发表时间: 2012-05-01
• 期刊: PROSTATE
• 影响因子: 2.8
• 作者: Endoh, Kanji;Nishi, Mayuko;Ryo, Akihide
• 通讯作者: Ryo, Akihide