Identification of endogenous SID-1 ligands

内源 SID-1 配体的鉴定

• 批准号: 21390063
• 负责人: MITANI Shohei
• 金额: $ 8.24万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390063/
• 关键词: 分子; 細胞生理学; 線虫; RNA干渉; SID-1; RNAi伝播; RNAi; リガンド

RNAi(RNA interference) is now a popular method to downregulate gene functions of interest. In C. elegans, it has been known that dsRNAs transmit from cells to other cells. This phenomenon is called as systemic RNAi and SID-1 protein is the representative gene that is required for dsRNA uptake in systemic RNAi. We searched for molecules involved in the phenomenon and found that animals are resistant to gon-1 RNAi in the vps-45 mutant background. GON-1 protein is a metalloprotease and necessary for secretion of various proteins from distal tip cells and body wall muscle cells. We found that GON domain alone is important for this secretion.

RNAi（RNA干扰）现在是下调感兴趣的基因功能的流行方法。在秀丽隐杆线虫中，众所周知，DSRNA从细胞传播到其他细胞。这种现象称为全身性RNAi，而SID-1蛋白是全身RNAi中DsRNA摄取所必需的代表性基因。我们搜索了参与该现象的分子，发现动物在VPS-45突变体背景下对GON-1 RNAi具有抗性。 GON-1蛋白是一种金属蛋白酶，是远端尖端细胞和体壁肌肉细胞分泌各种蛋白所必需的。我们发现，仅GON域对于此分泌很重要。

项目成果

Functional analysis of GS28, an intra-Golgi v-SNARE

高尔基体内 v-SNARE GS28 的功能分析

• 发表时间: 2009
• 期刊: C. elegans Genes to Cells
• 作者: Maekawa M;Inoue T;Kobuna H;Nishimura T;Gengyo-Ando K;Mitani S and Arai H
• 通讯作者: Mitani S and Arai H

National BioResource Project for the Nematode

国家线虫生物资源项目

• 发表时间: 2010
• 作者: Hashimoto H;et al.;S.Mitani
• 通讯作者: S.Mitani

Combinatorial regulation of three TRPM channels governs magnesium homeostasis

三个 TRPM 通道的组合调节控制镁稳态

• 发表时间: 2010
• 期刊: PLoS One
• 影响因子: 3.7
• 作者: Teramoto T;Sternick LA;Kage-Nakadai E;Sajjadi S;Siembida J;Mitani S;Iwasaki K and Lambie EJ
• 通讯作者: Iwasaki K and Lambie EJ

GPI anchor synthesis in the distal tip cells is indispensable for development and differentiation of germline cells of the nematode Caenorhabditis elegans

远端细胞中的 GPI 锚合成对于线虫生殖细胞的发育和分化是必不可少的

• 发表时间: 2012
• 期刊: Mol. Biol. Cell
• 作者: Murata D;Nomura KH;Dejima K;Mizuguchi S;Kawasaki N;Matsuishi-Nakajima Y;Ito S;Gengyo-Ando K;Kage-Nakadai E;Mitani S and Nomura K
• 通讯作者: Mitani S and Nomura K

Phosphorylation of α-synuclein at Ser129 reduces neuronal dysfunction by lowering its membrane-binding property in Caenorhabditis elegans

α-突触核蛋白 Ser129 的磷酸化通过降低秀丽隐杆线虫的膜结合特性来减少神经元功能障碍

• 发表时间: 2012
• 期刊: J Biol Chem.
• 作者: Kuwahara T;Tonegawa R;Mitani S;Iwatsubo T
• 通讯作者: Iwatsubo T