What dietary supplementation can acclimate broiler chickens to heat stress?

哪些膳食补充剂可以使肉鸡适应热应激？

• 批准号: 21380165
• 负责人: TOYOMIZU Masaaki
• 金额: $ 11.48万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380165/
• 关键词: 暑熱ストレス; 地球温暖化対策; ミトコンドリア; 抗酸化飼料; ニワトリ; 活性酸素

The objective of this study was to find a possible method to improve growth performance of broiler chickens exposed to chronic heat stress. 1)Mitochondrial ROS production for control group showed little changes during the experimental periods, whereas that for the heat-stressed group (34°C)was increased after 3, 5 and 9 d of heat exposure and returned to original levels at 14d. 2)Myoblasts isolated from the pectoralissuperficialis muscles of 0-1 day old chicks were cultured and exposed to 41°C for 6 h, resulting in increased ROS production and reduced protein content of the cells. These changes were attenuated by pretreatment ofan antioxidant, Tempol for 1 h. 3)Supplementation of glutamine and glutamate to basal diets slightly but definitely alleviated decreases in body weight gain, food intake and, and ratio of the duodenal villus height to crypt depth of heat-stressed broiler chickens. Further biochemical studies including modifications of intestinal function are required to establish a method for alleviation of the stress.

本研究的目的是寻找一种可能的方法来改善慢性热应激下肉鸡的生长性能 1) 实验期间对照组的线粒体 ROS 产生几乎没有变化，而热应激组 (34) 的线粒体 ROS 产量几乎没有变化。 °C)在热暴露3、5和9天后增加，并在14天时恢复到原始水平2)从0-1天的胸浅肌中分离的成肌细胞。老雏鸡在 41°C 下培养 6 小时，导致细胞中 ROS 产生增加，蛋白质含量降低，这些变化可通过抗氧化剂 Tempol 预处理 1 小时来减弱。 3) 补充谷氨酰胺和谷氨酸。基础日粮略微但确实缓解了热应激肉鸡的体重增加、食物摄入量以及十二指肠绒毛高度与隐窝深度之比的下降。需要进行包括肠道功能改变在内的生化研究来建立缓解压力的方法。

项目成果

急性暑熱にともなう鶏骨格筋におけるROS過剰産生機構の解明

阐明急性热导致鸡骨骼肌ROS过量产生的机制

• 发表时间: 2010
• 期刊: 家畜栄養生理研究会報
• 作者: Ueda;W.;M. Tohno;T. Shimazu;H. Fujie;H. Aso;Y. Kawai;M. Numasaki;T. Saito;H. Kitazawa;工藤亮治・増本隆夫・吉田武郎・堀川直紀;小松崎将一;杉山修一;喜久里基
• 通讯作者: 喜久里基

Meat-type chickens have a higher efficiency of mitochondrial oxidative phosphorylation than laying-type chickens

• DOI: 10.1016/j.cbpa.2011.01.020
• 发表时间: 2011-05-01
• 期刊: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
• 影响因子: 2.3
• 作者: Toyomizu, Masaaki;Kikusato, Motoi;Amo, Taku
• 通讯作者: Amo, Taku

Mitochondrial membrane potential decrease caused by loss of PINK1 isnot due to proton leak, not to respiratory chain defects

PINK1 缺失引起的线粒体膜电位降低不是由于质子泄漏，也不是由于呼吸链缺陷

• 发表时间: 2010
• 期刊: Neurobiol.Dis.
• 作者: Amo;T.;Sato;S.;Saiki;S.;Wolf;A.M.;Toyomizu;M.;Gautier;C.A.;Shen;L.;Ohta;S.;Hattori;N.
• 通讯作者: N.

Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks

• DOI: 10.1016/j.cbpa.2012.03.009
• 发表时间: 2012-07-01
• 期刊: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
• 影响因子: 2.3
• 作者: Chowdhury, Vishwajit S.;Tomonaga, Shozo;Furuse, Mitsuhiro
• 通讯作者: Furuse, Mitsuhiro

慢性暑熱下における鶏小腸粘膜のミトコンドリア代謝応答の組織特異性ならびにグルタミンおよびグルタミン酸同時給与による暑熱ストレス緩和作用の検証

慢性热下鸡小肠粘膜线粒体代谢反应的组织特异性及同时饲喂谷氨酰胺和谷氨酸缓解热应激效果的验证

• 发表时间: 2012
• 作者: Escudero E;et al;Miwa Y;中山泰史
• 通讯作者: 中山泰史