Ecological study on marine luminous bacteria possessing proteorhodopsin

具有蛋白视紫红质的海洋发光细菌的生态学研究

• 批准号: 21380123
• 负责人: KOGURE Kazuhiro
• 金额: $ 11.4万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380123/
• 关键词: プロテオロドプシン; 海洋細菌; 光エネルギー; プロトンポンプ活性; Flavobacteria; 遺伝子解析; 遺伝子伝搬; 16S rDNA; Bacteroidetes; エルギー代謝; 海洋生態系; 系統解析; エネルギー代謝

Proteorhodopsin (PR) is a membrane protein that absorbs light energy and functions as proton pump. The proton gradient thus established enables the cells to synthesize ATP, thus serves as efficient energy synthesis component. This was first reported in 2000. Since then there have been estimated that nearly half of prokaryotes present in the upper water column in the ocean may possess PR. However, so far only about 10 isolates had been reported to possess PR, that makes any further physiological investigations difficult. Our group succeeded to isolate more than 60 strains and directly measure the activity of proton pump under light. In addition, with further assumptions we succeeded to estimate the contribution of PR to light energy absorption to the ocean for the first time. The research on PR is now expanding after our investigation using isolates.

蛋白质淡淡蛋白酶（PR）是一种膜蛋白，可吸收光能并用作质子泵。因此，质子梯度可以使细胞合成ATP，因此可以作为有效的能量合成成分。这是在2000年首次报道的。从那时起，据估计，海洋上水柱中存在近一半的原核生物可能具有PR。但是，到目前为止，只有大约10个分离株具有PR，这使得任何进一步的生理研究变得困难。我们的小组成功地隔离了60多个菌株，并直接测量了在光下质子泵的活性。此外，有了进一步的假设，我们成功地估算了PR对光吸收对海洋的贡献。在我们使用分离株进行调查后，对PR的研究正在扩展。

项目成果

VNCの概念の変遷と新たな展開

VNC概念的变化和新发展

• 发表时间: 2011
• 作者: 佐藤一道;澤辺智雄;木暮一啓
• 通讯作者: 木暮一啓

Halioglobus japonicus gen. nov., sp. nov., and Halioglobus pacificus sp. nov., within the class Gammaproteobacteria, isolated from seawater

日本盐球菌 (Halioglobus japonicus)

• 发表时间: 2012
• 期刊: International Journal of Systematics and Evolutionary Microbiology(Doi: 10.1099/ijs.0.031443-0)
• 作者: Park;Sanghwa;Susumu Yoshizawa;Kentaro Inomata;Kazuhiro Kogure and Akira Yokota
• 通讯作者: Kazuhiro Kogure and Akira Yokota

Exploring marine microbial unknown functions

探索海洋微生物的未知功能

• 发表时间: 2012
• 作者: 平井慈恵;小磯雅彦;照屋和久;奥澤公一;小林真人;武部孝行;佐藤琢;中村航;後藤敬行;萩原篤志;木暮一啓
• 通讯作者: 木暮一啓

"Photoheterotrophy" -an overlooked mechanism of marine bacteria

“光异养”——海洋细菌被忽视的机制

• 发表时间: 2011
• 作者: Nishizawa;T.;et al.;橘本緑・白木原国雄・白木原美紀;Kogure Kazuhiro
• 通讯作者: Kogure Kazuhiro

Evidence for particle-induced horizontal gene transfer and serial transduction between bacteria

• DOI: 10.1111/j.1574-6941.2011.01077.x
• 发表时间: 2011-06-01
• 期刊: FEMS MICROBIOLOGY ECOLOGY
• 影响因子: 4.2
• 作者: Chiura, Hiroshi Xavier;Kogure, Kazuhiro;Velimirov, Branko
• 通讯作者: Velimirov, Branko