Research on Symbiotic Bacteria in Capitella sp.1

Capitella sp.1共生菌的研究

• 批准号: 08456093
• 负责人: KOGURE Kazuhiro
• 金额: $ 4.86万
• 依托单位: Ocean Research Institute, The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456093/
• 关键词: Capitella sp.1; Heterotrophic Bacteria; Bacterial Activity; Organic Pollution; イオウ化合物; 有機物代謝; イオウ細菌; 環境浄化; 底質; 独立栄養; 共生細菌

Capitella sp. I is a bcnthic organism of which normal habitat is coastal polluted sediments. Previous observations clarified that presence of this organism may prevent from further deterioration of the environments. The actual mechanism was assumed to be introduction of oxic seawater from upper water column and oxidation of sulfide to sulfur or sulfate. Bacteria may play an important role as nutritional source for Capitella sp. I and/or degrader of organic pollutants in the environments. The purpose of this investigation was to clarify these contributions. During the course of this study, we developed a new method to culture and observe the behavior of Capitella sp. I directly, by using transparent containcr and solidified agar. In addition, we used the chemical (I NT) which made it possible to visualize and quantity the bacterial respiratory activity. The result showed that Capitella sp. I stimulates the activity of bacteria by making holes in the sediment and introducing oxic seawater. As heterotrophic bacterial activity is assumed to couple to degradation of organic matter, it was conchided that presence and behavior of Capitella sp. I does play important roles to prevent from deterioration. This new technique will be applicable for various research on the interact on between benthic organisms and bacteria.

Capitella sp。我是一种BCNTHIC生物体，正常栖息地是沿海污染的沉积物。先前的观察结果阐明了这种生物的存在可能会阻止环境进一步恶化。假定实际机制是从上水柱和硫化物氧化到硫酸盐或硫酸盐的氧化引入。细菌可能是Capitella sp的营养来源的重要作用。 I和/或环境中有机污染物的降解者。这项调查的目的是澄清这些贡献。在本研究过程中，我们开发了一种新方法来培养和观察Capitella sp的行为。我直接使用透明的包含和固化的琼脂。此外，我们使用了化学（I NT），使细菌呼吸活性可视化和数量成为可能。结果表明Capitella sp。我通过在沉积物中制造孔并引入毒性海水来刺激细菌的活性。由于假定异养的细菌活性将其赋予有机物的降解，因此可以说明Capitella sp的存在和行为。我确实扮演着重要的角色，以防止恶化。这项新技术将适用于底栖生物与细菌之间相互作用的各种研究。