Proteins of an entomopoxvirus : its proteomic analysis and a study for the development of technology for the use of its protein fusolin as a synergist of viral insecticides

昆虫痘病毒蛋白：其蛋白质组学分析及利用其蛋白fusolin作为病毒杀虫剂增效剂的技术开发研究

• 批准号: 21380044
• 负责人: MITSUHASHI Wataru
• 金额: $ 12.56万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380044/
• 关键词: 昆虫ポックスウイルス; 核多角体病ウイルス; ゲノム解析; プロテオーム解析; 酵母外来遺伝子発現系; 代替え宿主; 立体構造; ドウガネブイブイ; 酵母発現系

The genome of an alphaentomopoxvirus virus was completely sequenced for the first time. The genome of the virus that infects a coleopteran, Anomala cuprea(AcEPV) is ca. 246 kb in length, which is smaller than those of Alphaentomopoxvirus previously considered, and consists of a central coding region bounded by inverted terminal repeats(ITR) that is the longest ITRs among wild type of poxviruses reported. The genome harbors ca. 250 open reading frames. An ORF which contains a serine protease inhibitor(serpins) domain sequence, which may harbor an immunological function in mammalians not insects was detected. Fusolin gene, which expresses a viral enhancing factor, was introduced into an expression system of foreign genes in yeast for the purpose of large amount of the production of the protein for the development of a system for the enhancement of virus insecticides.

首次完全测序了字母瘤病毒病毒的基因组。感染了鞘翅目，Anomala Cuprea（ACEPV）的病毒的基因组。长度为246 kb，比以前考虑的αenentomopoxvirus的长度小，由一个由倒末端重复序列（ITR）界定的中央编码区组成，该区域是野生类型的POXVIRUSS中最长的ITR。基因组港口大约。 250个开放式阅读帧。一个包含丝氨酸蛋白酶抑制剂（SERPINS）结构域的ORF，该序列可能在哺乳动物非昆虫中具有免疫功能。表达病毒增强因子的富索蛋白基因被引入酵母中外源基因的表达系统，目的是为了开发蛋白质的大量产生，以开发一种增强病毒杀虫剂的系统。

项目成果

Recent advances in studies for the application of a protein produced by entomopoxviruses(Poxviridae) for insect-pest control

昆虫痘病毒（痘病毒科）产生的蛋白质在害虫防治中的应用研究进展

• 发表时间: 2009
• 期刊: JARQ
• 作者: Yukuhiro K;Iwata K;Komoto N;Tomita S;Kadono K;Kiuchi M;Itoh M;Banno Y;西大海・飯山和弘・青木智佐・清水進;Mitsuhashi W
• 通讯作者: Mitsuhashi W

Recent advances in studies for the application of a protein produced by entomopoxviruses (Poxviridae) for insect-pest control

昆虫痘病毒（痘病毒科）产生的蛋白质在害虫防治中的应用研究进展

• 发表时间: 2009
• 期刊: Japan Agricultural Research Quarterly (JARQ) 43
• 作者: Fujii;T. 他7名;W.Mitsuhashi
• 通讯作者: W.Mitsuhashi