The regulation of cell proliferation and adhesion by chloride channel related factor CLCA.

氯通道相关因子 CLCA 对细胞增殖和粘附的调节。

• 批准号: 21592407
• 负责人: OKAMURA Kazuhiko
• 金额: $ 2.5万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592407/
• 关键词: イオンチャネル; CLCA; 細胞増殖; 分化; 癌の浸潤; クロライドチャネル; 発生・分化; 細胞接着; siRNA抑制; 再構築モデル; p63

A rat CLCA(originally named chloride channels, calcium-activated) homologue fCLCA has a splicing isoform trCLCA. Owing to the weak association of trCLCA to the other CLCA isoforms of other species, the morphological and functional significance of trCLCA has not been established. We have carried out the tissue localization of trCLCA in rat, with support of grants-in-aid for Scientific Research(C) 18592025.Subsequently, functional analyses of trCLCA was undertaken in this project, using in vitro system of reconstruction model of stratified squamous epithelium and several kinds of cultured cells. One of important result is : inhibition of trCLCA expression by siRNA bring out the change of cytokeratin profile, followed by negative regulation of cellular adhesion and proliferation.

大鼠 CLCA（最初称为氯离子通道，钙激活）同源物 fCLCA 具有剪接亚型 trCLCA。由于 trCLCA 与其他物种的其他 CLCA 亚型的关联较弱，因此 trCLCA 的形态和功能意义尚未确定。在科学研究资助基金(C)18592025的支持下，我们在大鼠体内进行了trCLCA的组织定位。随后，本项目利用复层鳞状细胞重建模型体外系统对trCLCA进行了功能分析。上皮和几种培养细胞。重要结果之一是：siRNA抑制trCLCA表达引起细胞角蛋白谱的变化，进而负向调节细胞粘附和增殖。

项目成果

Transformation from fibroblast to myofibroblast is regulated by TGFβ and mechanical tension in a rat skin reconstruction model

大鼠皮肤重建模型中成纤维细胞向肌成纤维细胞的转化受 TGFβ 和机械张力的调节

• 发表时间: 2012
• 作者: Hata S;Okamura K;Ishikawa H;Yamazaki J
• 通讯作者: Yamazaki J

Blockade of TRPM8 activity reduces the invasion potential of oral squamous carcinoma cell lines

• DOI: 10.3892/ijo.2012.1340
• 发表时间: 2012-05-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Okamoto, Yoshihiko;Ohkubo, Tsuyako;Yamazaki, Jun
• 通讯作者: Yamazaki, Jun

Expression of caspase-14 in stratified and cornified epithelium in a ratoral mucosal reconstruction model

口腔粘膜重建模型中复层和角化上皮中 caspase-14 的表达

• 发表时间: 2012
• 作者: Murakami H;Okamura K;Sakagami R;Yamazaki J
• 通讯作者: Yamazaki J

CLCA isoforms and structural proteins differentially expressed in a reconstuction model derived from rat skin

大鼠皮肤重建模型中 CLCA 亚型和结构蛋白的差异表达

• 发表时间: 2012
• 作者: Yamazaki J;Okamura K;Murakami H;Hata S;et al
• 通讯作者: et al