Molecular mechanism of hypoxia-induced genome instability in human colorectal cancer

缺氧引起人类结直肠癌基因组不稳定的分子机制

• 批准号: 21591736
• 负责人: HEMMI Hiromichi
• 金额: $ 3.08万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591736/
• 关键词: 遺伝子; 大腸がん; ゲノム不安定性; EMAST; 発現制御; 低酸素; DNA修復; GLUT1

Frequency of EMAST, a phenotype of genetic instability, in Japan was approximately 60%, which is similar to USA. Down-regulation of hMSH3 by hypoxia was studied in human cell lines derived from different origins including colon. Three patterns : rapid, moderate, and none responses were observed. Moderate type was observed in cells having mutant p53.Histochemical staining of clinical samples supports that down-regulation of hMSH3 is induced by hypoxia. Transient hypoxia is not enough for generation of EMAST tumor. Repeated states of hypoxia-reoxyganation may require for induction of EMAST.

EMAST（一种遗传不稳定表型）在日本的频率约为 60%，与美国相似。在来自不同来源（包括结肠）的人类细胞系中研究了缺氧对 hMSH3 的下调。观察到三种模式：快速、中等和无反应。在具有突变p53的细胞中观察到中型。临床样品的组织化学染色支持hMSH3的下调是由缺氧诱导的。短暂的缺氧不足以产生 EMAAST 肿瘤。反复缺氧-复氧状态可能需要诱导 EMAST。

项目成果

Effluent markers related to epithelial mesenchymal transition with adjusted values for effluent cancer antigen 125 in peritoneal dialysis patients.

• DOI: 10.4061/2011/261040
• 发表时间: 2011
• 期刊: International journal of nephrology
• 影响因子: 2.1
• 作者: Mizuiri S;Hemmi H;Arita M;Tai R;Hattori Y;Muto A;Suzuki Y;Ohashi Y;Sakai K;Aikawa A
• 通讯作者: Aikawa A

Transcriptional regulation of the hMLH1 gene by CXXC5, a zinc finger protein

锌指蛋白 CXXC5 对 hMLH1 基因的转录调控

• 发表时间: 2009
• 作者: 有田通恒;李傑;菅野新一郎;安井明;逸見仁道
• 通讯作者: 逸見仁道

A study in regulatory mechanism of the MSH3 gene expression

MSH3基因表达调控机制的研究

• 发表时间: 2009
• 作者: Li J;Min Z;Arita M;Koi M;Hemmi H
• 通讯作者: Hemmi H

CXXC5とSYF/p29によるhM転写制御, a zinc finger protein

CXXC5 和 SYF/p29（一种锌指蛋白）的 hM 转录调节

• 发表时间: 2010
• 作者: 有田通恒;李傑;菅野新一郎;安井明;逸見仁道
• 通讯作者: 逸見仁道

14-3-3σ-dependent resistance to cisplatin

14-3-3σ 依赖性顺铂耐药

• 发表时间: 2009
• 期刊: Anticancer Res
• 影响因子: 2
• 作者: Han Z;Dimas K;Tian X;Wang Y;Hemmi H;Yamada K;Kato N;Pantazis P;Ramanujam RJ;Anant S;Wyche JH;Houchen CW
• 通讯作者: Houchen CW