Research on correlations between the intracellular composition of fatty acids and the protein degradation machinery of insulin secretion-related enzymes.

细胞内脂肪酸组成与胰岛素分泌相关酶的蛋白质降解机制的相关性研究。

• 批准号: 21580163
• 负责人: ANDO Hideya
• 金额: $ 3.08万
• 依托单位: Okayama University of Science (2011)Doshisha University (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580163/
• 关键词: 酵素; 細胞; 組織; 脂質; 蛋白質分解; 糖尿病; 細胞・組織; 脂肪酸; インシュリン; 培養細胞; ユビキチン化; タンパク質分解

The effects of alterations of the intracellular composition of fatty acids on insulin secretion by cultured murine pancreatic β cells(MIN6 cells) was evaluated. The insulin secretion from MIN6 cells was enhanced most efficiently by oleic acid, followed by linoleic acid and α-linolenic acid, when each fatty acid was treated transiently at the same molar concentration. On the other hand, preincubation with each fatty acid for 72 hr in advance changed the intracellular composition of fatty acids and the most efficient effect of insulin secretion under this condition was elicited by α-linolenic acid. These results suggest that the insulin secreting activity of cultured MIN6 cells is affected by the intracellular composition of fatty acids.

评估了脂肪酸细胞内组成对培养的鼠胰腺β细胞（MIN6细胞）胰岛素分泌的影响。当每种脂肪酸以相同的摩尔浓度暂时处理时，用油酸和α-烯醇酸的油酸和α-烯醇酸最有效地增强了来自MIN6细胞的胰岛素分泌。另一方面，提前对每种脂肪酸进行72小时的预孵育改变了脂肪酸的细胞内组成，在这种情况下，胰岛素分泌的最有效效果是由α-烯丙基酸引起的。这些结果表明，培养的MIN6细胞的胰岛素分泌活性受脂肪酸的细胞内组成的影响。

项目成果

培養膵β細胞のインスリン分泌作用に及ぼす長時間遊離脂肪酸処理の影響

长期游离脂肪酸处理对培养胰腺β细胞胰岛素分泌的影响

• 发表时间: 2011
• 作者: 安藤秀哉;福島光夫;高橋良寿;今井緑;寺師浩人;清野進;市橋正光
• 通讯作者: 市橋正光

Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area

• DOI: 10.1111/j.1755-148x.2009.00640.x
• 发表时间: 2010-02-01
• 期刊: PIGMENT CELL & MELANOMA RESEARCH
• 影响因子: 4.3
• 作者: Ando, Hideya;Niki, Yoko;Ichihashi, Masamitsu
• 通讯作者: Ichihashi, Masamitsu

Inhibition of melanin synthesis via modification of tyrosinase function

通过修饰酪氨酸酶功能抑制黑色素合成

• 发表时间: 2009
• 作者: Ando H;Ichihashi M;Hearing VJ.
• 通讯作者: Hearing VJ.

1-(2, 4-Dihydroxyphenyl)-3-(2, 4-dimethoxy-3-methylphenyl) propane inhibits melanin synthesis by dual mechanisms

1-(2, 4-二羟基苯基)-3-(2, 4-二甲氧基-3-甲基苯基)丙烷通过双重机制抑制黑色素合成

• 发表时间: 2011
• 期刊: Journal of Dermatological Science
• 影响因子: 4.6
• 作者: Niki Y;Yoshida M;Ando H;Wakamatsu K;Ito S;Harada N;Matsui M;Yarosh D;Ichihashi M.
• 通讯作者: Ichihashi M.

A new proposal for the evaluation method of melanosome transfer in respect to melanosome uptake by keratinocyte phagocytosis

关于角质形成细胞吞噬黑素体摄取的黑素体转移评估方法的新建议

• 发表时间: 2009
• 作者: Ando H;Niki Y;Yoshida M;Matsui M;Ichihashi M.
• 通讯作者: Ichihashi M.