Identification of glycosyltransferases that regulate morphology and differentiation of filamentous fungus

调节丝状真菌形态和分化的糖基转移酶的鉴定

• 批准号: 21580096
• 负责人: GOTO Masatoshi
• 金额: $ 3.16万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580096/
• 关键词: 糸状菌; Aspergillus; O-結合型糖鎖; 糖転移酵素

Aspergillus nidulans possesses three putativeα-1, 2-mannosyltransferases(Mnt) that are involved in protein O-glycosylation. We attempted to identify and characterize three Mnts of A. nidulans. The structure of O-glycans and mannosyltransferase activity using wt strain and three mnt-disruptants were determined. A. nidulans mnts(Anmnts) respectively expressed in a triple mnt mutant of yeast. The results indicate that AnMntA and AnMntC are not involved in O-glycosylation unlike to yeast Mnts, and that AnMntB functions in part similar to yeast Mnts. We determined whether AnMnts catalyze the synthetic reaction of the cell wall component, galactomannan that is present in Aspergilli. The results indicate that three AnMnts may be involved in synthesis ofα-1, 2-mannan polymer in galactomannan.

todentif y和三个Mnts的nidulans。在类似于酵母菌的部分中，我们确定ANMNT是否催化了细胞壁成分ULTS，表明三个ANMNT可能参与了半乳糖中的α-1，2-Mannan聚合物的合成。

项目成果

Functional analysisof glycosylated Wsc proteins involved in cell wall integrity

参与细胞壁完整性的糖基化 Wsc 蛋白的功能分析

• 发表时间: 2010
• 作者: T. Futagami;Y. Kajiwara;H. Takashita;T. Omori;M. Goto
• 通讯作者: M. Goto

Aspergillus nidulansにおけるmannosyltransferase遺伝子群の機能解析

构巢曲霉甘露糖基转移酶基因的功能分析

• 发表时间: 2009
• 作者: 井上大輔;大橋貴生;二神泰基;大森俊郎;竹川薫;後藤正利
• 通讯作者: 後藤正利

Aspergillus nidulansにおけるkre9ホモログ遺伝子の機能解析

构巢曲霉kre9同源基因的功能分析

• 发表时间: 2011
• 作者: 上原拓磨;瀬戸和史;二神泰基;大森俊郎;竹川薫;後藤正利
• 通讯作者: 後藤正利

Quest for the genes encoding O-glycosylated proteins that are responsible for the maintenance of cell morphology in Aspergillus nidulans

寻找编码 O-糖基化蛋白的基因，这些蛋白负责构巢曲霉细胞形态的维持

• 发表时间: 2011
• 作者: M.Goto;K.Seto;T.Futagami;Y.Kajiwara;H.Takashita;T.Omori;K.Takegawa.
• 通讯作者: K.Takegawa.

麹菌のポストゲノム研究の展開:糸状菌に特異な機能未知遺伝子を探る

曲霉菌后基因组研究进展：探索丝状真菌特有的未知功能基因

• 发表时间: 2011
• 期刊: 日本生物工学会誌
• 作者: 後藤正利;岩下和裕
• 通讯作者: 岩下和裕