Resonance Raman Study on the Proton Pumping Mechanism of Cytochrome c Oxidase

细胞色素c氧化酶质子泵浦机制的共振拉曼研究

• 批准号: 21570171
• 负责人: OGURA Takashi
• 金额: $ 2.91万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570171/
• 关键词: 好気呼吸; 生体エネルギー変換; チトクロムC酸化酵素; 酸素還元; プロトンポンプ; 共鳴ラマン; 部位特異的アミノ酸置換; 振動スペクトル; ヘム; 水素結合; ミトコンドリア; 細胞呼吸; チトクロムc酸化酵素; 酸素活性化; タンパク質構造ダイナミクス; 酸化的リン酸化; 呼吸鎖電子伝達系; 構造ダイナミクス; 鉄-ヒスチジン結合

Cytochrome c oxidase plays an essentially important function in cell respiration to maintain life, and catalyzes dioxygen reduction to water, which is coupled with proton pumping across the membrane. In order to elucidate the mechanism of this enzyme, high resolution structures at a picometer level are to be determined by resonance Raman spectroscopy. In the present study, we have established assignments of some Raman siganals. In addition, an intermediate conformational state and cooperative dynamics of the two hemes have been detected, which are essential for an efficient proton pump.

细胞色素 c 氧化酶在维持生命的细胞呼吸中发挥着至关重要的作用，并催化双氧还原成水，这与跨膜的质子泵送相结合。为了阐明这种酶的机制，需要通过共振拉曼光谱来确定皮米水平的高分辨率结构。在本研究中，我们建立了一些拉曼信号的分配。此外，还检测到了两种血红素的中间构象状态和协同动力学，这对于高效质子泵至关重要。

项目成果

Site-directed isotope labeling of cytochrome c oxidase

细胞色素c氧化酶的定点同位素标记

• 发表时间: 2009
• 作者: Hideo Shimada;Yukie Katayama;Takashi Ogura;Tomitake Tsukihara;Shinya Yoshikawa
• 通讯作者: Shinya Yoshikawa

Protein Dynamics of Cytochrome c Oxidase upon CN-Photolysis as Studied w ith Resonance Raman Spectroscopy

用共振拉曼光谱研究细胞色素 c 氧化酶在 CN 光解作用下的蛋白质动力学

• 发表时间: 2011
• 作者: Takeshi Nishigaki;et al.
• 通讯作者: et al.

Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein

• DOI: 10.1007/s10863-010-9285-8
• 发表时间: 2010-06-01
• 期刊: JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
• 影响因子: 3
• 作者: Katayama, Yukie;Shimokata, Kunitoshi;Shimada, Hideo
• 通讯作者: Shimada, Hideo

Distinct binding geometries of CO to L-Trp- and D-Trp-bound indoleamine 2,3-dioxygenase as probed with resonance Raman spectroscopy

用共振拉曼光谱探测 CO 与 L-Trp 和 D-Trp 结合吲哚胺 2,3-双加氧酶的独特结合几何形状

• 发表时间: 2010
• 作者: Sachiko Yanagisawa;et al.
• 通讯作者: et al.

Microsecond Protein Dynamics of Cytochrome c Oxidase as Studied with Resonance Raman Spectroscopy

共振拉曼光谱研究细胞色素 c 氧化酶的微秒蛋白质动力学

• 发表时间: 2011
• 作者: T. Ogura;I. Ishigami;T. Nishigaki;S. Nakashima;K. Shinzawa-Itoh and Shinya Yoshikawa
• 通讯作者: K. Shinzawa-Itoh and Shinya Yoshikawa