SUBRETNAL TRANSPLANTION OF HUMAN AMNIOTIC MEMBRANE IN RABBITS

兔人羊膜的视网膜下移植

基本信息

批准号：
12671725
负责人：
IWASAKI Takuya
金额：
$ 1.92万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Submacular surgery for the removal of choroidal neovasucularization in age related macular degeneration can improve or sstabilize vision in selected partients. However, the retinal pigment epithelium are frequently damaged and contribute to the poor visual outcome. Human amniotic membrane is thick basement membrane and has anti-adhesive properties and promote epithelialization and decrease inflammation, neovasucularization, and fibrosis. Amniotic membrane transplantation has been reported to be effective in the reconstruction of the ocular surface. Using rabbit model, we have investigated the reconstruction of retinal pigment epithelium following subretinal implantation of human amniotic membrane. The implantation of amniotic membrane was achieved using standard subretinal surgery and instrument. Weekly examination was performed and 1 to 4 weeks after implantation, rabbits were sacrificed for histological and immune histological study of CD4 and CD8. No evidence of inflammation, fibrin, retinal edema, retinal detachment was detected. The retina reattached over the amniotic membrane. Histological cvaluation revealed PE proliferation under the amniotic membrane in 2 weeks. However, PE proliferation did not observed over bare Bruch's membrane. The CD4 and CD8 positive cells were observed in 2 weeks, but decreased in 4 weeks. The tolerance of subretinal human amniotic membrane in rabbits showed both amniotic membrane and subretinal space are immune-privileged site. The amniotic membrane may serve as a useful replacement during submacular surgery.

黄斑下手术切除年龄相关性黄斑变性中的脉络膜新生血管可以改善或稳定某些患者的视力。然而，视网膜色素上皮经常受损并导致视觉效果不佳。人羊膜是厚的基底膜，具有抗粘连特性，促进上皮化，减少炎症、新生血管形成和纤维化。据报道，羊膜移植可有效重建眼表。利用兔模型，我们研究了人羊膜视网膜下植入后视网膜色素上皮的重建。使用标准视网膜下手术和仪器实现羊膜植入。每周进行检查，植入后1至4周处死兔子进行CD4和CD8的组织学和免疫组织学研究。未检测到炎症、纤维蛋白、视网膜水肿、视网膜脱离的证据。视网膜重新附着在羊膜上。组织学评估显示 2 周内羊膜下 PE 增殖。然而，在裸露的布鲁赫膜上没有观察到PE增殖。 CD4和CD8阳性细胞在2周内可见，但在4周后减少。兔视网膜下人羊膜的耐受性表明羊膜和视网膜下腔都是免疫豁免部位。羊膜可以作为黄斑下手术期间有用的替代物。