Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions

低温条件下拟南芥根部磷脂生物合成位点的可视化

• 批准号: 21570034
• 负责人: NISHIDA Ikuo
• 金额: $ 3.16万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21570034/
• 关键词: ホスファチジルコリン; ホスファチジルエタノールアミン; 低温応答; リン脂質合成; 根の伸長; 表皮細胞の内膜構造; CDP-コリン合成; ホスファチジルセリン; リン脂質合成のしくみ; 根の表皮細胞; 内膜構造

WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.

WS，CCT2，CCT1-1和CCT1-1 CCT2植物以降序表现出CCT活性。 CCT1-1 CCT2幼苗无法在2处伸长根。根表皮细胞在环境温度下在WS，CCT2和CCT1和CCT1-1幼苗中表现出ER-Tracker荧光，在2或在盐胁迫下膨胀。相比之下，内膜结构在环境温度下在CCT1-1 CCT2幼苗中发育不足，在冷或盐压力幼苗中消失。 SGFP-CCT1和SGFP-CCT2荧光与转基因拟南芥根中的ER-Tracker荧光有关。这些结果表明，拟南芥根中应力诱导的ER发育和细胞伸长所必需的功能CCT。

项目成果

(1)低温でリン脂質ホスファチジルコリンが生合成されるしくみ

(1)磷脂酰胆碱低温生物合成机理

Unusual phenotypes of Arabidopsis mutants defective in phospholipid biosynthesis

磷脂生物合成缺陷的拟南芥突变体的异常表型

• 发表时间: 2010
• 作者: Nishida;I
• 通讯作者: I

Tpect1-4 mutation decreases leaf respiration byaffecting the mitochondrial cytochrome pathway capacity

Tpect1-4 突变通过影响线粒体细胞色素途径能力来降低叶片呼吸

• 发表时间: 2011
• 作者: Otsuru M.;Yu Y.;Mizoi J.;Fujiki Y.;Nishida I.
• 通讯作者: Nishida I.

The effects of pect1-4mutation on the lipid composition and the respiration activity of isolated mitochondria

pect1-4突变对分离线粒体脂质组成和呼吸活性的影响

• 发表时间: 2010
• 作者: Yu;Y.;Kawai;H.;Kawamoto;M.;Mizoi;J.;Fujiki;Y.;Nishida;I.
• 通讯作者: I.

The importance of PHOSPHATIDYLSERINE SYNTASE1 in root development of Arabidopsis thaliana

磷脂酰丝氨酸合酶1在拟南芥根系发育中的重要性

• 发表时间: 2011
• 作者: Yamaoka Y.;Duan Z.;Fujiki Y.;Nishida I.
• 通讯作者: Nishida I.