CLINICOPATHOLOGIC STUDIES FOR HISTOLOGIC GRADE AND PROGNOSIS OF SYNOVIAL SARCOMA

滑膜肉瘤组织学分级和预后的临床病理学研究

• 批准号: 12670188
• 负责人: HASEGAWA Tadashi
• 金额: $ 1.02万
• 依托单位: NATIONAL CANCER CENTER
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670188/
• 关键词: SYNOVIAL SARCOMA; HISTOLOGIC GRADE; MIB-1; β-CATENIN; FUSION GENE TRANSCRIPTS; LIPOSARCOMA; VALIDITY; REPRODUCIBILITY; 組織学的悪性度評価; β型カテニン遺伝子; 成人神経芽腫; 脱分化脂肪肉腫; 紡錘形細胞腫瘍; 悪性骨腫瘍; 類骨骨腫; 細胞間接着蛋白

1) We foundhistologic grade using MIB-1score was themost important prognostic factor in dedifferentiated liposarcomas of the retroperitoneum and synovial sarcomas.2) We studied immunoreactivity for β-catenin, a cadherin undercoat protein, in 44 cases of synovial sarcoma. We found that nuclear expression of β-catenin, that was present in 57% of cases, was associated with an adverse outcome.3) We examined for the presence of tumor-specific fusion gene transcripts by a reverse transcription polymelase chain reaction assay using RNA samples from the frozen tumor tissue of synovial sarcoma and adult neuroblastoma, and found molecular diagnosis was successfully performed by this analysis.4) We examined the validity and reproducibility of diagnosis of histologic type and of 2 grading systems for adult soft-tissue sarcomas by comparing independent diagnoses of 4 pathologists and the diagnosis of the expert panel at National Cancer Center We obtained the results that diagnosis of the type of soft-tissue sarcoma for synovial sarcoma, small round-cell sarcoma, and liposarcoma and the MIB-1 grading system based on tumor differentiation were highly valid and reproducible among pathologists.

1) 我们发现使用 MIB-1 评分的组织学分级是腹膜后去分化脂肪肉瘤和滑膜肉瘤中最重要的预后因素。2) 我们在 44 例滑膜肉瘤中研究了 β-连环蛋白（一种钙粘蛋白下层蛋白）的免疫反应性。 β-连环蛋白的核表达存在于 57% 的病例中，与不良结果相关。3) 我们检查了使用滑膜肉瘤和成体神经母细胞瘤冷冻肿瘤组织的 RNA 样本，通过逆转录聚合酶链反应测定肿瘤特异性融合基因转录本的存在，并发现通过该分析成功地进行了分子诊断。4）我们检查了有效性通过比较 4 位病理学家的独立诊断和国家癌症中心专家小组的诊断，我们获得了以下结果：滑膜肉瘤、小圆细胞肉瘤和脂肪肉瘤的软组织肉瘤类型诊断以及基于肿瘤分化的 MIB-1 分级系统在病理学家中具有高度的有效性和可重复性。

项目成果

Kimura K., Hasegawa T., et al.: "Germline p53 mutation in a patient with multiple primary cancers"Jpn J Clin Oncol. 31(7). 349-351 (2001)

Kimura K.、Hasekawa T.等人：“患有多种原发性癌症的患者中的种系 p53 突变”Jpn J Clin Oncol。

Takahiro Kasamatsu, Tadashi Hasegawa, et al: "Primary epithelioid sarcoma of vulva"Int J Gynecol cancer. 11(4). 316-320 (2001)

Takahiro Kasamatsu、Tadashi Hasekawa 等人：“外阴原发性上皮样肉瘤”Int J Gynecol cancer。

Yoji Kawaguchi, Tadashi Hasegawa, et al: "Mechanism of intramedullary high intensity area on T2-weighted magnetic resonance imaging inosteoidosteoma : a possible role of COX-2 expression"Pathol Int. 51(12). 933-937 (2001)

Yoji Kawaguchi、Tadashi Hasekawa 等人：“T2 加权磁共振成像骨内骨瘤髓内高强度区域的机制：COX-2 表达的可能作用”Pathol Int。

Okada K., Hasegawa T., et al.: "Rosette-forming epithelioid osteosarcoma : a histologic subtype with a highly aggressive clinical behavior"Hum Pathol. 32(7). 726-733 (2001)

Okada K.、Hasekawa T. 等人：“玫瑰花结型上皮样骨肉瘤：具有高度侵袭性临床行为的组织学亚型”Hum Pathol。

Minoru Tanaka, Tadashi Hasegawa, et al: "Minoru Tanaka, Tadashi Hasegawa, et al"Minoru Tanaka, Tadashi Hasegawa, et al. 92(2). 338-341 (2000)

田中稔、长谷川正等人：“田中稔、长谷川正等人”田中稔、长谷川正等人。