Gene therapy using sonoporation for maxillofacial distraction

使用声孔进行颌面牵引的基因治疗

• 批准号: 20592340
• 负责人: YOSHIOKA Izumi
• 金额: $ 2.91万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592340/
• 关键词: 顎骨延長法; BMP; Smad; ヒアルロン酸; 骨芽細胞; 超音波遺伝子導入; マイクロバブル; ソノポレーション法

Bone morphogenetic protein-2 (BMP-2) is expected to be utilized to fill bone defects and promote healing of fractures. However, it is unable to generate an adequate clinical response for use in bone regeneration. Recently, it was reported that glycosaminoglycans, including heparin, heparan sulfate, keratan sulfate, dermatan sulfate, chondroitin-4-sulfate, chondroitin-6-sulfate, and hyaluronic acid (HA), regulate BMP-2 activity, though the mechanism by which HA regulates osteogenic activities has not been fully elucidated. The aim of this study was to investigate the effects of HA on osteoblast differentiation induced by BMP-2.Monolayer cultures of osteoblastic lineage MG63 cells were incubated with BMP-2 and HA for various time periods. To determine osteoblastic differentiation, alkaline phosphatase (ALP) activity in the cell lysates was quantified. Phosphorylation of Smad 1/5/8, p38, and ERK proteins was determined by Western blot analysis. To elucidate the nuclear translocation of phosphorylated Smad 1/5/8, stimulated cells were subjected to immunofluorescence microscopy. To further elucidate the role of HA in enhancement of BMP-2-induced Smad signaling, mRNA expressions of the BMP-2 receptor antagonists noggin and follistatin were detected using real-time RT-PCR.BMP-2-induced ALP activation, Smad 1/5/8 phosphorylation, and nuclear translocation were up-regulated when MG63 cells were cultured with both BMP-2 and HA. Western blot analysis revealed that phosphorylation of ERK protein was diminished by HA. Furthermore, the mRNA expressions of noggin and follistatin induced by BMP-2 were preferentially blocked by HA.These results indicate that HA enhanced BMP-2 induces osteoblastic differentiation in MG63 cells via down-regulation of BMP-2 antagonists and ERK phosphorylation.

骨形态发生蛋白2（BMP-2）有望用来填充骨缺损并促进裂缝的愈合。但是，它无法产生足够的临床反应以用于骨再生。最近，据报道，包括肝素，硫酸乙酰肝素，硫酸肝素，硫酸盐，硫酸皮肤菌，软骨素-4-硫酸盐，硫酸软骨蛋白-4-硫酸盐，6-硫酸软骨蛋白-6-硫酸盐和软骨酸（HA），透明质酸（HA），尽管Elucitiiss的机械性均未进行机械性，而Haegulates Incogenisis Incogenical sogeniciss ost ost oste，包括肝素，硫酸乙酸乙酰胺，硫酸盐，硫酸盐，硫酸盐。这项研究的目的是研究HA对BMP-2诱导的成骨细胞分化的作用。将成骨细胞谱系MG63细胞的单层培养物与BMP-2和HA孵育不同。为了确定成骨细胞分化，定量细胞裂解物中的碱性磷酸酶（ALP）活性。 SMAD 1/5/8，P38和ERK蛋白的磷酸化通过Western印迹分析确定。为了阐明磷酸化SMAD的核易位1/5/8，刺激的细胞进行免疫荧光显微镜。为了进一步阐明HA在增强BMP-2诱导的SMAD信号传导中的作用，使用实时RT-PCR.BMP-2诱导的ALP激活，BMP-2受体拮抗剂Noggin和Follistatin的mRNA表达，SMAD 1/5/8 PhosphoryLation和核转运在Mg63细胞上均与HA BRM时，SMAD 1/5/8 phosploration corportation smad 1/5/8 phosperation诱导的ALP激活。 Western印迹分析表明，HA会减少ERK蛋白的磷酸化。此外，BMP-2诱导的Noggin和Follistatin的mRNA表达优先通过HA来阻止。这些结果表明，HA增强的BMP-2通过下调BMP-2拮抗剂和ERK磷酸化而诱导MG63细胞中成骨细胞分化。

项目成果

顎関節症の疾患概念と治療概念

颞下颌关节紊乱病的疾病概念及治疗理念

• 发表时间: 2009
• 期刊: 九州歯会誌 63
• 作者: Furuta;H.;冨永和宏
• 通讯作者: 冨永和宏

A comparison of stability following mandibular setback between vertical ramus and sagittal split osteotomies

垂直升支截骨术与矢状劈裂截骨术下颌后缩后稳定性的比较

• 发表时间: 2008
• 期刊: Journal of Oral and Maxillofac Surg 66
• 作者: Kazuoki Hayashi;Izumi Yoshioka;Norihiko Furuta;Amit Khanal;Kazuhiro Tominaga;Khanal A;Izumi Yoshioka
• 通讯作者: Izumi Yoshioka

Correlation of mandibular bone quality with neurosensory disturbance after sagittal split ramus Osteotomy

升支矢状劈裂截骨术后下颌骨骨质量与感觉神经障碍的相关性

• 发表时间: 2010
• 期刊: British J Oral Maxillofac Surg
• 作者: Izumi Yoshioka;他
• 通讯作者: 他

CT画像での下顎管の位置と下顎枝矢状分割術後の下唇オトガイ部知覚異常との関係

CT图像中下颌管位置与下颌升支矢状分割后下唇、颏区感觉异常的关系

• 发表时间: 2009
• 作者: 吉岡泉;副島和久;井川加織;鹿嶋光司;迫田隅男;吉岡泉
• 通讯作者: 吉岡泉

馬蹄形骨切りを併用した Le Fort I型骨切り術による上顎骨後方移動術の臨床的検討

Le Fort I截骨联合马蹄形截骨治疗上颌后移的临床研究

• 发表时间: 2009
• 作者: 吉岡泉;副島和久;井川加織;鹿嶋光司;迫田隅男;吉岡泉;吉岡泉
• 通讯作者: 吉岡泉