Examination to an amelogenin gene expression in several tissues in a body

检查体内多个组织中的牙釉蛋白基因表达

基本信息

批准号：
20592235
负责人：
YAMADA Yoshishige
金额：
$ 3.08万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592235/
关键词：
アメロジェニン免疫組織染色ラット切歯マウス胎児アメロジェニン遺伝子歯関組織以外の臓器胎生18齢生後8週齢 PCR cDNA In situ LAMP法免疫組織学染色法 In situ PCRIn LAMP In situ LAMP 免疫染色

项目摘要

The gene expression of amelogenin in several mouse tissues were evaluated in this study. The samples were chosen from embryonic 9day, 18day mouse tissue mix cDNA, 8week mouse tissues mix cDNA and several each target tissues cDNA. The target tissues for this study were selected 16 tissues (brain, heart, lung, liver, stomach, uterus, placenta, kidney, pancreas, testine, muscle, skin, small intestine, large intestine, spleen, and ovary)from 8week old mouse body. The polymerase chain reaction(PCR)was used to amplify several size of amelogenin using a part of exon6 to exon7(small size of PCR amplification : 226bp).The result of PCR amplification showed the positive react of gene expression at 8week mouse tissues mix cDNA and 18day mouse tissue mix cDNA. However none of gene expression was detected by embryonic 9 day mouse tissues mix cDNA. The result of the PCR amplification for 16tissues was obtained amelogenin gene expression in 14 tissues excluded stomach and pancreas. These results suggested that amelogenin genes are expressed in various tissues and this expression is not only restricted to development stage that also expressed in adult mouse. These results suggested that amelogenin might play some homeostatic role in various tissues of the body.

在这项研究中评估了几只小鼠组织中氨基蛋白蛋白的基因表达。从胚胎9天，18天小鼠组织混合cDNA，8周小鼠组织混合cDNA和几种目标组织cDNA中选择样品。这项研究的目标组织选择了16个组织（大脑，心脏，肺，胃，胃，子宫，胎盘，肾脏，胰腺，胰腺，睾丸，肌肉，皮肤，小肠，大肠，脾脏，脾和卵巢）身体。聚合酶链反应（PCR）使用Exon6的一部分到Exon7（PCR扩增的小尺寸：226bp）来扩增几种大小的amelogen蛋白。PCR扩增的结果表明，基因表达在8week小鼠组织中的基因表达在8week小鼠组织中呈阳性反应。和18天小鼠组织混合cDNA。但是，没有通过胚胎9天小鼠组织混合cDNA检测到基因表达。在不包括胃和胰腺的14个组织中，获得了16个虫的PCR扩增的结果。这些结果表明，阿米他素基因在各种组织中表达，并且该表达不仅限于在成年小鼠中也表达的发育阶段。这些结果表明，氨基蛋白酶可能在人体各种组织中发挥某些稳态作用。