Mass spectrometer (LC-MS)

质谱仪（LC-MS）

• 批准号: 537496341
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/537496341?language=en
• 关键词: Mass; spectrometer; LC; MS

This grant application is for a highly sensitive mass spectrometer including a high-performance liquid chromatography system and associated software for the analytics of drugs and endogenous metabolites. Besides the quantification of known drugs, endogenous metabolites and peptides, this system will also support the qualitative identification of structurally so far unknown molecules. The mass spectrometry facility at the Chair of Clinical Pharmacology and Clinical Toxicology is developing and applying quantitative methods for a wide variety of small endogenous metabolites and drugs in different matrices in own research projects as well as in co-operations. The requested system is supposed to replace an old mass spectrometer, that went out of service, and therefore new analytical capacities should be generated. The focus lies on a liquid chromatography mass spectrometer system with high analytical sensitivity in order to achieve low limits of detection and quantification, which are well below those of mass spectrometers currently available at the mass spectrometry facility. Besides the afore mentioned highly sensitive quantification, the new mass spectrometer will expand the present possibilities in the context of untargeted metabolomics. One of the biggest challenges lies in the correct identification of detected signals. The requested mass spectrometer can analyze fragmentation patterns with an additional layer of fragmentation, which constitutes as an important aid for structure elucidation, and hence can help to increase the validity of untargeted metabolomics analyses. Furthermore, it is planned to use commercially available kits for targeted metabolomics with the requested system to complement untargeted metabolomics analyses.

该赠款应用于高度敏感的质谱仪，包括用于药物和内源代谢物分析的高性能液相色谱系统和相关软件。除了定量已知药物，内源性代谢产物和肽外，该系统还将支持迄今为止未知分子的定性鉴定。临床药理学和临床毒理学主席的质谱设施正在开发并应用定量方法，用于在自己的研究项目以及合作中的不同基质中的各种小型内源代谢物和药物。所请求的系统应该替换旧的质谱仪，该质谱仪无法使用，因此应生成新的分析能力。重点放在具有高分析灵敏度的液相色谱质谱仪系统上，以达到较低的检测和定量限制，该系统远低于当前在质谱设施处可用的质谱仪的质谱。除了上述高度敏感的量化外，新的质谱仪还将在未靶向的代谢组学的背景下扩展当前的可能性。最大的挑战之一在于正确识别检测到的信号。请求的质谱仪可以用额外的碎片分析碎裂模式，这构成了结构阐明的重要辅助，因此可以帮助提高未靶向的代谢组学分析的有效性。此外，计划将商用套件与请求的系统用于有针对性的代谢组学来补充非目标的代谢组学分析。